Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6, citrate buffer, for 10-20 min
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 50 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 37°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Cell signaling anti-Rabbit (H&L) IgG HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Aug 23 2021