Recombinant Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free
See all CD8 alpha primary antibodies -
Description
Rabbit monoclonal [EPR20305] to CD8 alpha - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment aa 1-200. The exact sequence is proprietary.
Database link: P01731 -
Positive control
- WB: Mouse lymph node, thymus and spleen lysates. IHC-P: Mouse spleen and thymus tissues.
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General notes
Ab228856 is the carrier-free version of ab209775. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab228856 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20305 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab228856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD8 antibody
- CD8 antigen alpha polypeptide antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)](https://www.abcam.com/ps/products/228/ab228856/Images/ab228856-309448-anti-cd8-alpha-antibody-epr20305-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)This IHC data was generated using the same anti-CD8 alpha antibody clone, EPR20305, in a different buffer formulation (cat# ab209775).
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on mouse thymus. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)](https://www.abcam.com/ps/products/228/ab228856/Images/ab228856-309447-anti-cd8-alpha-antibody-epr20305-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] - BSA and Azide free (ab228856)This IHC data was generated using the same anti-CD8 alpha antibody clone, EPR20305, in a different buffer formulation (cat# ab209775).
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on T cells around peripheral artery lymphatic sheath of mouse spleen (PMID: 23482450). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
References
ab228856 has not yet been referenced specifically in any publications.
