Key features and details
- Mouse monoclonal [OX-8] to CD8 alpha
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-CD8 alpha antibody [OX-8]
See all CD8 alpha primary antibodies
DescriptionMouse monoclonal [OX-8] to CD8 alpha
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Rat
Tissue, cells or virus. High molecular weight rat thymocyte glycoproteins
- WB: Rat thymus tissue lysate. IHC-P: Rat Spleen. Flow Cyt: Rat splenocytes. ICC/IF: Rat Spleenocytes cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityImmunogen affinity purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab33786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.|
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
ab33786 staining CD8 alpha in Rat Spleenocytes cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab33786 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 100% methanol (5 min).
IHC image of CD8 alpha staining in a section of formalin-fixed paraffin-embedded normal Rat Spleen performed on a Leica BOND™ system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab33923, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-CD8 alpha antibody [OX-8] (ab33786) at 1 µg/ml
Lane 1 : Rat thymus tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab33786 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at a 1 μg/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with 10% neutral buffered formalin and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then permeabilized with Triton X-100, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab33786 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a biotin-conjugated rabbit anti-mouse polyclonal, used at a 1/300 dilution.
Flow cytometry staining of Lewis rat splenocytes with ab33786 (right) or mouse IgG1 kappa; (ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33786) or mouse IgG1 kappa; (ab170190) isotype (1x 106 in 100 µl at 0.008 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD4.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live CD3 positive T cells.
ab33786 has been referenced in 17 publications.
- De Oliveira Andrade F et al. Effects of Jaeumkanghwa-tang on tamoxifen responsiveness in preclinical ER+ breast cancer model. Endocr Relat Cancer 26:339-353 (2019). PubMed: 30640711
- Wang M et al. Aberrant accumulation of Dickkopf 4 promotes tumor progression via forming the immune suppressive microenvironment in gastrointestinal stromal tumor. Cancer Med 8:5352-5366 (2019). PubMed: 31353847
- Mauseth B et al. The Novel Oncolytic Compound LTX-401 Induces Antitumor Immune Responses in Experimental Hepatocellular Carcinoma. Mol Ther Oncolytics 14:139-148 (2019). PubMed: 31211244
- Li W et al. Integrated tumor stromal features of hepatocellular carcinoma reveals two distinct subtypes with prognostic/predictive significance. Aging (Albany NY) 11:4478-4509 (2019). PubMed: 31299011
- Tahta A et al. Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turk Neurosurg 28:571-581 (2018). PubMed: 30192361