Overview

  • Product name
    Anti-CD8 alpha antibody [OX-8]
    See all CD8 alpha primary antibodies
  • Description
    Mouse monoclonal [OX-8] to CD8 alpha
  • Host species
    Mouse
  • Tested applications
    Suitable for: Blocking, Flow Cyt, IHC-Fr, IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat thymocyte membrane glycoproteins.

  • Positive control
    • IHC-Fr: Rat lymph node and spleen tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    OX-8
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Images

  • ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with 10% neutral buffered formalin and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then permeabilized with Triton X-100, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab33786 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a biotin-conjugated rabbit anti-mouse polyclonal, used at a 1/300 dilution.

    See Abreview

  • Frozen rat lymph node tissue stained for CD8 alpha using ab33786 (B, green) in immunohistochemical analysis. Also stained for CD11b (A, red). C is the merged image with nuclei counterstained blue using DAPI.

  • Frozen rat lymph node tissue stained for CD8 alpha using ab33786 (B, green) in immunohistochemical analysis. Also stained for CD25 (A, red). C is the merged image with nuclei counterstained blue using DAPI.

  • ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in acetone, blocked with 5% serum for 30 minutes at 25°C, then incubated with ab33786 at a 1/200 for 1 hour 30 minutes at 25°C. The secondary used was a biotin conjugated rabbit anti-mouse polyclonal, used at a 1/300.

    See Abreview

  • Frozen rat lymph node tissue stained for CD8 alpha using ab33786 (B, green) in immunohistochemical analysis. Also stained for CD25 (red). Nuclei counterstained blue using DAPI.

References

This product has been referenced in:
  • Menendez-Castro C  et al. Neonatal nephron loss during active nephrogenesis - detrimental impact with long-term renal consequences. Sci Rep 8:4542 (2018). IHC ; Rat . Read more (PubMed: 29540722) »
  • Iturra PA  et al. Progression of Type 2 Helper T Cell-Type Inflammation and Airway Remodeling in a Rodent Model of Naturally Acquired Subclinical Primary Pneumocystis Infection. Am J Pathol 188:417-431 (2018). IHC ; Rat . Read more (PubMed: 29169991) »

See all 8 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spleen)
Specification
spleen
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spleen)
Specification
spleen
Fixative
10 % Neutral Buffered Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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