Anti-CD8 alpha antibody [OX-8] (ab33786)
- Datasheet
- References (12)
- Protocols
Overview
-
Product nameAnti-CD8 alpha antibody [OX-8]
See all CD8 alpha primary antibodies -
DescriptionMouse monoclonal [OX-8] to CD8 alpha
-
Host speciesMouse
-
Tested applicationsSuitable for: IHC-P, WBmore details
-
Species reactivityReacts with: Rat
-
Immunogen
Tissue, cells or virus. High molecular weight rat thymocyte glycoproteins
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityMonoclonal
-
Clone numberOX-8
-
MyelomaNS1
-
IsotypeIgG1
-
Light chain typekappa
-
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab33786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. |
Target
-
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
-
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
-
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
-
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 24930 Rat
- SwissProt: P07725 Rat
- Unigene: 10306 Rat
-
Alternative names
- alpha polypeptide (p32) antibody
- CD8 antibody
- CD8 antigen alpha polypeptide antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [OX-8] (ab33786)](https://www.abcam.com/ps/products/33/ab33786/Images/ab33786-47204-anti-cd8-alpha-antibody-ox-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [OX-8] (ab33786)Image courtesy of an anonymous Abreview.ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with 10% neutral buffered formalin and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then permeabilized with Triton X-100, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab33786 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a biotin-conjugated rabbit anti-mouse polyclonal, used at a 1/300 dilution.
Protocols
References
This product has been referenced in:
- De Oliveira Andrade F et al. Effects of Jaeumkanghwa-tang on tamoxifen responsiveness in preclinical ER+ breast cancer model. Endocr Relat Cancer 26:339-353 (2019). Read more (PubMed: 30640711) »
- Maimon BE et al. Optogenetic Peripheral Nerve Immunogenicity. Sci Rep 8:14076 (2018). Read more (PubMed: 30232391) »
