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  1. Link

    cd8-alpha-antibody-yts1694-ab22378.pdf

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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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Anti-CD8 alpha antibody [YTS169.4] (ab22378)

  • Datasheet
  • SDS
Reviews (14)Q&A (9)References (68)

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Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)
  • Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)

Key features and details

  • Rat monoclonal [YTS169.4] to CD8 alpha
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-CD8 alpha antibody [YTS169.4]
    See all CD8 alpha primary antibodies
  • Description

    Rat monoclonal [YTS169.4] to CD8 alpha
  • Host species

    Rat
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Mouse spleen cells.
  • General notes

    ab22378 is not expected to work in standard western blots, under denaturing conditions, or in IHC on formalin-fixed tissue. To our knowledge, ab22378 will only stain frozen sections or fresh cells, and will only immunoprecipitate the antigen from cell lysates in mild detergents such as NP40 or Triton-X100.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    YTS169.4
  • Isotype

    IgG2b
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cancer
    • Tumor immunology
    • CD markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-CD8 alpha antibody [YTS169.4] - BSA and Azide free (ab230292)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab22378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 0.1-1µg for 106 cells.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Notes
Flow Cyt
Use 0.1-1µg for 106 cells.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01732 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12525 Mouse
    • SwissProt: P01731 Mouse
    • Unigene: 1858 Mouse
    • Alternative names

      • alpha polypeptide (p32) antibody
      • CD_antigen=CD8a antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly 2 antibody
      • Ly 35 antibody
      • Ly B antibody
      • Ly2 antibody
      • Ly3 antibody
      • Ly35 antibody
      • LyB antibody
      • Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T lymphocyte differentiation antigen T8/Leu 2 antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-cell surface glycoprotein Lyt 2 antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    Images

    • Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)
      Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)

      Normal mouse (CBA) spleen cells (red cell lysed) double stained with ab22378 (0.3 µg/mL, detected with Alexa Fluor® 488-conjugated monoclonal mouse anti-rat antibody) and an APC conjugated hamster anti-mouse CD3 antibody. ab22378 stains an expected subpopulation of CD8 alpha positive T cells.

    • Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)
      Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)

      Overlay histogram showing mouse spleen cells (C57BL/6) labeling CD8 alpha with ab22378 (red line). The cells were incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22378, 0.1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H&L) (ab150157) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x106 cells) used under the same conditions.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (68)

    Publishing research using ab22378? Please let us know so that we can cite the reference in this datasheet.

    ab22378 has been referenced in 68 publications.

    • Chen S  et al. Chemerin-9 Attenuates Experimental Abdominal Aortic Aneurysm Formation in ApoE-/- Mice. J Oncol 2021:6629204 (2021). PubMed: 33953746
    • Wang Z  et al. Disruption of the HER3-PI3K-mTOR oncogenic signaling axis and PD-1 blockade as a multimodal precision immunotherapy in head and neck cancer. Nat Commun 12:2383 (2021). PubMed: 33888713
    • Zhang H  et al. Augmenting ATG14 alleviates atherosclerosis and inhibits inflammation via promotion of autophagosome-lysosome fusion in macrophages. Autophagy N/A:1-13 (2021). PubMed: 33849389
    • Zhao Y  et al. Generation of triacyl lipopeptide-modified glycoproteins by metabolic glycoengineering as the neoantigen to boost anti-tumor immune response. Theranostics 11:7425-7438 (2021). PubMed: 34158858
    • He Q  et al. A Systemic Prime-Intrarectal Pull Strategy Raises Rectum-Resident CD8+ T Cells for Effective Protection in a Murine Model of LM-OVA Infection. Front Immunol 11:571248 (2020). PubMed: 33072113
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 23 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD8 alpha antibody [YTS169.4]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
    Permeabilization
    No
    Specification
    tumor
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 23 2022

    Immunohistochemistry (Frozen sections) abreview for Anti-CD8 alpha antibody [YTS169.4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Intestine, Spleen)
    Permeabilization
    No
    Specification
    Intestine, Spleen
    Blocking step
    10% Normal Serum, 1% BSA, 0.3 M Glycine in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Brett Bell

    Verified customer

    Submitted Oct 21 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD8 antibody [YTS169.4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain bearing GL261 tumor)
    Antigen retrieval step
    None
    Specification
    brain bearing GL261 tumor
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Francoise Geffroy

    Verified customer

    Submitted Feb 18 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-CD8 antibody [YTS169.4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (tumor tissue)
    Permeabilization
    Yes - 5% Triton X-100 in PBS
    Specification
    tumor tissue
    Blocking step
    Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 27 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-CD8 antibody [YTS169.4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (MC38 tumor)
    Permeabilization
    Yes - 1x PBS with 0.3% triton-x-100
    Specification
    MC38 tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ravikumar Muthuswamy

    Verified customer

    Submitted May 11 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-CD8 [YTS169.4] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (skin wound)
    Specification
    skin wound
    Permeabilization
    No
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 28 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-CD8 antibody [YTS169.4]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Lymph Node)
    Specification
    Lymph Node
    Fixative
    Acetone/Alcohol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 19 2012

    Question

    One doubt: some of this antibody only react with mouse, and you suggested human tissue. Is it correct?
    Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 14 2012

    Answer

    Thank you for your email.

    There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections can used as positive control.

    Actually the positive control information for each antibody is present on the datasheet; please check the datasheet. Alternatively, we always follow human protein Atlas database (http://www.proteinatlas.org/index.php). The second option is, searching positive control for other antibodies in catalogue against same target; the cell line or tissue sections tested with those antibodies could also be used as positive control. The third place to search for is literature.

    I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Aug 15 2012

    Question

    Our customer would like to know which are the best positive control
    and negative control for the antibodies below.
    1. anti Natural killer - ab36392
    2. anti- interferon gama - ab9918
    3. anti TNF alfa - ab34674
    4. anti TGF beta - ab15537
    5. anti neutrófilo - ab2557
    6. anti CD20 - ab9475
    7. anti células T CD8 - ab22378
    8. anti células T CD4 - ab51312
    She will do ICC and IHC-P assays.
    Thanks and best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you for contacting us.

    The positive control for ab36392 is IL2 activatedcultured NK cells.

    ab9918; human tonsil sections, human cervical squamous cell carcinoma section

    ab34674; human artery or colon tissue sections

    ab15537; HepG2 cells. mouse brain sections, human placenta sections; Human hepatocellular liver carcinoma cell line; Human hepatocarcinoma tissue sections

    ab2557; mouse spleen tissue sections

    ab9475; human tonsil tissue sections

    ab22378; mouse spleen tissue sections

    ab51312; Thymus tissue sections, human pancreas tissue sections.

    The best database to search for positive control is Human Protein Atlas. Please click the link http://www.proteinatlas.org/index.php

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 10 2012

    Question

    Dear Sir

    I am a professor of Shandong university and I bought two antibodies a few days ago, which were anti-CD8 antibody [YTS169.4] (ab22378) and anti-CD86 antibody [GL-1] (ab119857). I want to use these two antibodies to detect the expression of CD8 and CD 86 in B16 melanoma, which was grown in the armpit of mice. The detection method is IHC-Fr, but there is no dilution radio for IHC-Fr http://www.iciba.com/detection in the instruction. Can you tell me the dilution radio of these two antibodies? I will like to thank you for giving me the help and I am look forward to your reply.

    Read More

    Abcam community

    Verified customer

    Asked on May 22 2012

    Answer

    Thank you for contacting us.

    We suggest to optimise the working concentration according to the sample. Initially, you could try using 1:250 for ab22378 and 2ug/ml for ab119857.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on May 22 2012

    1-10 of 23 Abreviews or Q&A

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