Key features and details
- Rat monoclonal [YTS169.4] to CD8 alpha
- Suitable for: ICC/IF, Flow Cyt, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2b
Product nameAnti-CD8 alpha antibody [YTS169.4]
See all CD8 alpha primary antibodies
DescriptionRat monoclonal [YTS169.4] to CD8 alpha
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Frmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
- Flow Cyt: Mouse spleen cells.
ab22378 is not expected to work in standard western blots, under denaturing conditions, or in IHC on formalin-fixed tissue. To our knowledge, ab22378 will only stain frozen sections or fresh cells, and will only immunoprecipitate the antigen from cell lysates in mild detergents such as NP40 or Triton-X100.
This antibody clone is manufactured by Abcam.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Our Abpromise guarantee covers the use of ab22378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.1-1µg for 106 cells.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
IHC-Fr labeling for CD8-positive T-Cells, as indicated by red immunofluorescence (ab22378 at a 1/50 dilution). Yellow arrows designate positive cells. *** = p≤0.001.
Frozen sections from mouse skin (8 µm thick) were post-fixed in cold acetone at −20°C for 10 minutes then air dried at room temperature for 20 minutes. All sections were then washed three times with 0.05% PBS-Tween (PBST); then nonspecific antibody binding was blocked using 10% normal goat serum in PBST at room temperature for 30 minutes. Sections were then incubated overnight at 4°C with primary antibody. Primary antibody incubation was followed by three washes with PBST before addition of the secondary antibodies, which were incubated on the sections for 2 hours at room temperature. Nuclei were counterstained with DAPI (200 ng/mL) for 10 minutes at room temperature. Finally, sections were rinsed with PBST, dehydrated through sequential alcohol washes and then cleared in xylene. Slides were mounted with DPX mounting medium.
Normal mouse (CBA) spleen cells (red cell lysed) double stained with ab22378 (0.3 µg/mL, detected with Alexa Fluor® 488-conjugated monoclonal mouse anti-rat antibody) and an APC conjugated hamster anti-mouse CD3 antibody. ab22378 stains an expected subpopulation of CD8 alpha positive T cells.
Overlay histogram showing mouse spleen cells (C57BL/6) labeling CD8 alpha with ab22378 (red line). The cells were incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22378, 0.1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H&L) (ab150157) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab22378 staining CD8 alpha in murine bone marrow cells by ICC/IF (immunocytochemistry/immunofluorescence).
Cells were formaldehyde fixed prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody (1/200) was incubated with the sample for 9 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated goat anti-rat polyclonal was used as the secondary. The nuclei were stained with DAPI.
ab22378 staining CD8 alpha in mouse spleen tissue sections by IHC-Fr (Frozen sections).
Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/250 in PBS-Tween) at 4°C for 12 hours. An Alexa Fluor® 594-conjugated Goat polyclonal to rat IgG (1/250) was used as secondary antibody. Nuclei were stained with DAPI.
ab22378 has been referenced in 47 publications.
- Wang HY et al. Autophagy inhibition via Becn1 downregulation improves the mesenchymal stem cells antifibrotic potential in experimental liver fibrosis. J Cell Physiol 235:2722-2737 (2020). PubMed: 31508820
- Lin W et al. Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity. Theranostics 10:4871-4884 (2020). PubMed: 32308755
- Niu J et al. Aberrant oligodendroglial-vascular interactions disrupt the blood-brain barrier, triggering CNS inflammation. Nat Neurosci 22:709-718 (2019). PubMed: 30988524
- Wang Z et al. Syngeneic animal models of tobacco-associated oral cancer reveal the activity of in situ anti-CTLA-4. Nat Commun 10:5546 (2019). PubMed: 31804466
- Zhou B et al. Disulfiram combined with copper induces immunosuppression via PD-L1 stabilization in hepatocellular carcinoma. Am J Cancer Res 9:2442-2455 (2019). PubMed: 31815045