Anti-CD8 alpha antibody [YTS169.4] (ab22378)
![Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)](https://www.abcam.com/ps/products/22/ab22378/Images/ab22378-31688-anti-cd8-antibody-yts1694-flow-cytometry.jpg "Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)")
Key features and details
- Rat monoclonal [YTS169.4] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
Overview
-
Product name
Anti-CD8 alpha antibody [YTS169.4]
See all CD8 alpha primary antibodies -
Description
Rat monoclonal [YTS169.4] to CD8 alpha -
Host species
Rat -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Flow Cyt: Mouse spleen cells.
-
General notes
ab22378 is not expected to work in standard western blots, under denaturing conditions, or in IHC on formalin-fixed tissue. To our knowledge, ab22378 will only stain frozen sections or fresh cells, and will only immunoprecipitate the antigen from cell lysates in mild detergents such as NP40 or Triton-X100.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
YTS169.4 -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.1-1µg for 106 cells.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 0.1-1µg for 106 cells. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
-
Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
-
Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)
Normal mouse (CBA) spleen cells (red cell lysed) double stained with ab22378 (0.3 µg/mL, detected with Alexa Fluor® 488-conjugated monoclonal mouse anti-rat antibody) and an APC conjugated hamster anti-mouse CD3 antibody. ab22378 stains an expected subpopulation of CD8 alpha positive T cells.
-
![Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)](https://www.abcam.com/ps/products/22/ab22378/Images/ab22378-199649-anti-cd8-antibody-yts1694-flow-cytometry.jpg)
Flow Cytometry - Anti-CD8 alpha antibody [YTS169.4] (ab22378)Overlay histogram showing mouse spleen cells (C57BL/6) labeling CD8 alpha with ab22378 (red line). The cells were incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22378, 0.1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H&L) (ab150157) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (68)
ab22378 has been referenced in 68 publications.
- Chen S et al. Chemerin-9 Attenuates Experimental Abdominal Aortic Aneurysm Formation in ApoE-/- Mice. J Oncol 2021:6629204 (2021). PubMed: 33953746
- Wang Z et al. Disruption of the HER3-PI3K-mTOR oncogenic signaling axis and PD-1 blockade as a multimodal precision immunotherapy in head and neck cancer. Nat Commun 12:2383 (2021). PubMed: 33888713
- Zhang H et al. Augmenting ATG14 alleviates atherosclerosis and inhibits inflammation via promotion of autophagosome-lysosome fusion in macrophages. Autophagy N/A:1-13 (2021). PubMed: 33849389
- Zhao Y et al. Generation of triacyl lipopeptide-modified glycoproteins by metabolic glycoengineering as the neoantigen to boost anti-tumor immune response. Theranostics 11:7425-7438 (2021). PubMed: 34158858
- He Q et al. A Systemic Prime-Intrarectal Pull Strategy Raises Rectum-Resident CD8+ T Cells for Effective Protection in a Murine Model of LM-OVA Infection. Front Immunol 11:571248 (2020). PubMed: 33072113