The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FACS: Use neat - 1/10.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
Zhang T et al. Cyclosporine a drug-delivery system for high-risk penetrating keratoplasty: Stabilizing the intraocular immune microenvironment. PLoS One13:e0196571 (2018).
Read more (PubMed: 29734357) »
Vitavska O et al. The V-ATPase subunit C binds to polymeric F-actin as well as to monomeric G-actin and induces cross-linking of actin filaments. J Biol Chem280:1070-6 (2005).
Read more (PubMed: 15525650) »