Key features and details
- Mouse monoclonal [12C7] to CD8 (FITC)
- Suitable for: Flow Cyt
- Reacts with: Rabbit
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-CD8 antibody [12C7] (FITC)
See all CD8 primary antibodies
DescriptionMouse monoclonal [12C7] to CD8 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityAb41323 recognises the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Rabbit
The details of the immunogen for this antibody are not available.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab41323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100 - 1/200.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
FormCD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
- CD8 antibody
- CD8 antigen, alpha polypeptide (p32) antibody
- CD8a antibody
ab41323 has been referenced in 2 publications.
- Zhang T et al. Cyclosporine a drug-delivery system for high-risk penetrating keratoplasty: Stabilizing the intraocular immune microenvironment. PLoS One 13:e0196571 (2018). PubMed: 29734357
- Vitavska O et al. The V-ATPase subunit C binds to polymeric F-actin as well as to monomeric G-actin and induces cross-linking of actin filaments. J Biol Chem 280:1070-6 (2005). PubMed: 15525650