Overview

  • Product name

  • Description

    Rabbit polyclonal to CD8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CD8 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101388)

  • Positive control

    • This antibody gave a positive signal in the following lysates: Human Thymus Lysate; Human Spleen Tissue; Human Tonsil Tissue; THP1 Whole Cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab85792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Form

    CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • Alternative names

    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

Images

  • All lanes : Anti-CD8 antibody (ab85792) at 1 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human tonsil normal tissue lysate - total protein (ab29615)
    Lane 4 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    CD8 is a glycoprotein which may account for the observed bands running at a higher molecular weight than predicted. Abcam welcomes customer feedback.
  • IHC image of CD8 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85792, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References

This product has been referenced in:

  • Roider J  et al. High-Frequency, Functional HIV-Specific T-Follicular Helper and Regulatory Cells Are Present Within Germinal Centers in Children but Not Adults. Front Immunol 9:1975 (2018). Read more (PubMed: 30258437) »
  • Maehara T  et al. Lesional CD4+ IFN-?+ cytotoxic T lymphocytes in IgG4-related dacryoadenitis and sialoadenitis. Ann Rheum Dis 76:377-385 (2017). Read more (PubMed: 27358392) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry ab85792. Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion. However, I have done a blast search and due to sequence homology ab85792 is expected to cross-react with two species with Pongo pygmaeus and Gorilla gorilla gorilla - but not with mouse. We have two alternative antibodies in our catalogue which cross-react with mouse CD8 i.e ab22378 and ab34282. I would suggest that your customers should take a look at the datasheets to see if they suit their need. Should you have any further questions, please do not hesitate to contact us.

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