Anti-CD8 alpha antibody [C8/144B], prediluted (ab75129)
Key features and details
- Mouse monoclonal [C8/144B] to CD8 alpha, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD8 alpha antibody [C8/144B], prediluted
See all CD8 alpha primary antibodies -
Description
Mouse monoclonal [C8/144B] to CD8 alpha, prediluted -
Host species
Mouse -
Specificity
We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD8 alpha (C terminal). A 13 amino acid synthetic peptide from the C terminal cytoplasmic domain.
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Epitope
C terminal -
Positive control
- Human tonsil and lymph node tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.09% Sodium azide
Constituent: 7.9% Tris HCl
Stabilizing agent. -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
C8/144B -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab75129 has been referenced in 7 publications.
- Tomiyama T et al. Myeloid-derived suppressor cell infiltration is associated with a poor prognosis in patients with hepatocellular carcinoma. Oncol Lett 23:93 (2022). PubMed: 35154424
- Smolle MA et al. T-regulatory cells predict clinical outcome in soft tissue sarcoma patients: a clinico-pathological study. Br J Cancer 125:717-724 (2021). PubMed: 34127811
- Annese T et al. Inflammatory Infiltrate and Angiogenesis in Mantle Cell Lymphoma. Transl Oncol 13:100744 (2020). PubMed: 32120334
- Kanno H et al. Differences in the immunosurveillance pattern associated with DNA mismatch repair status between right-sided and left-sided colorectal cancer. Cancer Sci 111:3032-3044 (2020). PubMed: 32449240
- Benkhoucha M et al. Identification of a novel population of highly cytotoxic c-Met-expressing CD8+ T lymphocytes. EMBO Rep 18:1545-1558 (2017). PubMed: 28751311
- Petukhova L et al. Genome-wide association study in alopecia areata implicates both innate and adaptive immunity. Nature 466:113-7 (2010). IHC-Fr ; Human . PubMed: 20596022
- Ratajczak P et al. Th17/Treg ratio in human graft-versus-host disease. Blood 116:1165-71 (2010). IHC-P ; Human . PubMed: 20484086