Overview

  • Product name
    Anti-CD8 antibody [MEM-31] (Allophycocyanin)
    See all CD8 primary antibodies
  • Description
    Mouse monoclonal [MEM-31] to CD8 (Allophycocyanin)
  • Host species
    Mouse
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications
    Suitable for: Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation: Crude thymus membrane fraction. (Human).

  • Epitope
    This antibody recognizes a conformationally dependent epitope of CD8 antigen.
  • General notes

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.

Properties

Applications

Our Abpromise guarantee covers the use of ab26004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Use 5-20 µl per 100 µl of whole blood.

 

 

 

ab91364 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in disease
      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similarities
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Form
      CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
    • Alternative names
      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    References

    This product has been referenced in:
    • Dieckmann NM  et al. Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes. Traffic 16:1330-41 (2015). Read more (PubMed: 26771955) »
    • Horejsí V  et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). Read more (PubMed: 2422063) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Thymus)
    Specification
    Thymus
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer PH6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

    Dr. R. Teng

    Verified customer

    Submitted May 03 2012

    Answer

    Thank you for submitting an Abreview of ab26004. As you probably have noticed, your review is going to bepublished very soon on our website.

    It was recently returned to you as we would like to know if the primary antibody was used diluted or undiluted before publishng it, and as this is important information for us and our customers.

    Since you obtained poor results using the antibody in a untested species and application, we would also like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

    Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

    - try using more primary antibody
    - try a different antigen retrieval method


    If you decide to follow these suggestions, please let me know if they are helpful. I look forward to your reply.

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Thymus)
    Specification
    Thymus
    Fixative
    Acetone
    Permeabilization
    Yes - Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

    Dr. R. Teng

    Verified customer

    Submitted May 02 2012

    Answer



    I am very pleased to hear you would like to accept our offer and testab18282, ab26004, and ab69764inIHC-P.You can just place the order for the 3 antibodies in the usual way you do it (through a purchaser, online, etc.). You do not need the codes for the first purchase, but you will need to mention them after you submit your Abreviews to get the free antibodies on your next order.


    These codes will give you 1 freeprimary antibody each before the expiration date. To redeem this offer, please submit Abreviews for IHC-Pand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreviews have been submitted and they can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for your inquiry, which was passed along from my colleague.


    We do have the Multicolor Human T cell marker panel, but as you know it is only tested in and guaranteed for FC.


    https://www.abcam.com/Multicolor-Human-T-cell-marker-panel-CD3-FITC-CD4-PE-CD8-APC-and-CD45-PE-Cy7-ab106073.html


    I have asked our New Products team if they would be interested in having you test this panel in IHC-P for the data, and we would reward you for that. I will let you know what they say.


    We currently don't sell the small aliquots of these antibodies separately. So alternatively, we can have you test the full sized vials of ab18282, ab26004, and ab69764 in IHC-P. Basically, you would purchase the 3 vials up front at cost, test them in IHC-P, submit your data to us in the form of 3 Abreviews (1 for each product), and you would receive 3 discount codes worth 3 free antibodies off your next Abcam purchase, even if the 3 antibodies fail in IHC-P. The discount codes are good for 4 months after issue.


    Let me know if you would be interested in testing these in IHC-P. I would be happy to answer any other questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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