Key features and details
- Mouse monoclonal [MEM-31] to CD8 (PerCP)
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2a
Product nameAnti-CD8 antibody [MEM-31] (PerCP)
See all CD8 primary antibodies
DescriptionMouse monoclonal [MEM-31] to CD8 (PerCP)
ConjugationPerCP. Ex: 482nm, Em: 675nm
Specificityab65949 recognizes a conformationally-dependent epitope of CD8. The antibody does not react with formaldehyde-fixed cells and is negative in Western Blotting.
Species reactivityReacts with: Human
Tissue/ cell preparation (Human):Crude thymus membrane fraction.
- human blood
Conjugated with Peridinin-chlorophyll-protein complex (PerCP)
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
Purification notes>95% by SDS-PAGE
Is unsuitable for WB.
Is unsuitable for applications using formaldehyde fixed cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsAll of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
FormCD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
- CD8 antibody
- CD8 antigen, alpha polypeptide (p32) antibody
- CD8a antibody
ab65949 staining CD86 in Human whole blood by Flow Cytometry. The sample was incubated with the primary antibody (1/50 in 2% Human serum + 0.5mM EDTA in PBS) for 25 minutes at 4°C.
Gating Strategy: Lymphocytes.
ab65949 has been referenced in 2 publications.
- Abu N et al. Subchronic toxicity, immunoregulation and anti-breast tumor effect of Nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L. BMC Complement Altern Med 18:31 (2018). PubMed: 29374471
- Linnebacher M et al. Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol 2010:841451 (2010). PubMed: 20339516