Anti-CD8 antibody [MEM-31] (PerCP/Cy5.5®) (ab157306)

Overview

  • Product name
    Anti-CD8 antibody [MEM-31] (PerCP/Cy5.5®)
    See all CD8 primary antibodies
  • Description
    Mouse monoclonal [MEM-31] to CD8 (PerCP/Cy5.5®)
  • Host species
    Mouse
  • Conjugation
    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Tested applications
    Suitable for: Flow Cyt, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Crude thymus membrane fraction.

  • Epitope
    ab157306 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.
  • General notes
    ab157306 does not react with formaldehyde-fixed cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab157306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells. (or 100 µl of whole blood).
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • Involvement in disease
      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • Sequence similarities
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Form
      CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
    • Alternative names
      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    Images

    • Surface staining of human peripheral blood using anti-human CD8 (clone MEM-31) PerCP-CyTM5.5.

    References

    ab157306 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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