FITC Anti-CD8 alpha antibody [SK1] (ab210367)
Key features and details
- FITC Mouse monoclonal [SK1] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD8 alpha antibody [SK1]
See all CD8 alpha primary antibodies -
Description
FITC Mouse monoclonal [SK1] to CD8 alpha -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, African green monkey -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Human peripheral blood lymphocytes.
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General notes
The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
This antibody is widely used as a phenotypic marker for CD8 alpha on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
SK1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 5µl for 105-8 cells.
in a final volume of approximately 100 µL |
Notes |
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Flow Cyt
Use 5µl for 105-8 cells. in a final volume of approximately 100 µL |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 101002639 Baboon
- Entrez Gene: 735755 Chimpanzee
- Entrez Gene: 102124337 Cynomolgus monkey
- Entrez Gene: 925 Human
- Entrez Gene: 698329 Rhesus monkey
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab210367 has not yet been referenced specifically in any publications.