Overview

  • Product name
    Anti-CD8 beta antibody [2ST8.5H7]
    See all CD8 beta primary antibodies
  • Description
    Mouse monoclonal [2ST8.5H7] to CD8 beta
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    Tissue, cells or virus corresponding to Human CD8 beta. Normal human T lymphocytes.

  • Epitope
    ab23616 binds to the CD8 beta chain, recognising an epitope that is dependent upon expression of both CD8 alpha and CD8 beta.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.5% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    2ST8.5H7
  • Myeloma
    NS1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Flow Cyt 1/1 - 1/10.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.

Target

  • Function
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
  • Tissue specificity
    Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Phosphorylated as a consequence of T-cell activation.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD8b antigen antibody
    • CD8 antigen beta polypeptide 1 (p37) antibody
    • CD8 antigen beta polypeptide 1 antibody
    • CD8 beta antibody
    • CD8b antibody
    • CD8b molecule antibody
    • CD8B_HUMAN antibody
    • CD8B1 antibody
    • Leu2 antibody
    • Ly3 antibody
    • LYT3 antibody
    • MGC119115 antibody
    • P37 antibody
    • T cell surface glycoprotein CD8 beta chain antibody
    • T lymphocyte surface glycoprotein beta chain antibody
    • T-cell surface glycoprotein CD8 beta chain antibody
    see all

References

This product has been referenced in:
  • Clement M  et al. Anti-CD8 Antibodies Can Trigger CD8+ T Cell Effector Function in the Absence of TCR Engagement and Improve Peptide-MHCI Tetramer Staining. J Immunol 187:654-63 (2011). Functional Studies . Read more (PubMed: 21677135) »
  • Yoshino N  et al. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim 49:97-110 (2000). Read more (PubMed: 10889948) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us. As discussed on the phone, this antibody is not conjugated and in order to detect it you would therefore either need a secondary antibody or to conjugate the antibody itself. We have several secondary antibodies to choose from and a very useful search engine in order to identify exactly what your looking for, allowing you to choose the conjugate, species and a number of other factors: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 If you would like help in choosing an appropriate antibody please do not hesitate to contact us. Alternatively you could directly conjugate the antibody itself using one of our conjugation kits: https://www.abcam.com/index.html?pageconfig=resource&rid=13148 Again, this can be used to attach the conjugate of your choice to the antibody for direct detection. I hope this information has been of help. If you have any further questions please do not hesitate to contact me again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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