Anti-CD8 beta antibody [H35-17-2] (ab269344)
Key features and details
- Rat monoclonal [H35-17-2] to CD8 beta
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
Overview
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Product name
Anti-CD8 beta antibody [H35-17-2]
See all CD8 beta primary antibodies -
Description
Rat monoclonal [H35-17-2] to CD8 beta -
Host species
Rat -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD8 beta. 5-day mixed leukocyte culture population, C57BL/6 anti-BALB/c
Database link: P10300 -
Positive control
- Flow Cyt: Murine splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
H35-17-2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. -
Tissue specificity
Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPhosphorylated as a consequence of T-cell activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12526 Mouse
- SwissProt: P10300 Mouse
- Unigene: 333148 Mouse
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Alternative names
- CD8b antigen antibody
- CD_antigen=CD8b antibody
- CD8 antigen 37 kDa chain antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab269344 has not yet been referenced specifically in any publications.