Anti-CD8 beta antibody [H35-17.2] (ab95768)
Key features and details
- Rat monoclonal [H35-17.2] to CD8 beta
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-CD8 beta antibody [H35-17.2]
See all CD8 beta primary antibodies -
Description
Rat monoclonal [H35-17.2] to CD8 beta -
Host species
Rat -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD8 beta. 5-day mixed leukocyte culture population, C57BL/6 anti-BALB/c
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Positive control
- BALB/c splenocytes; Mouse splenocytes and thymocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
H35-17.2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.25-0.5µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µl is recommended.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 0.25-0.5µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. -
Tissue specificity
Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPhosphorylated as a consequence of T-cell activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12526 Mouse
- SwissProt: P10300 Mouse
- Unigene: 333148 Mouse
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Alternative names
- CD8b antigen antibody
- CD_antigen=CD8b antibody
- CD8 antigen 37 kDa chain antibody
see all
Images
Datasheets and documents
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Datasheet download
References (1)
ab95768 has been referenced in 1 publication.
- Tarleton RL et al. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun 62:1820-9 (1994). PubMed: 8168945