Overview

  • Product name

    Anti-CD8 beta antibody [H35-17.2] (FITC)
    See all CD8 beta primary antibodies
  • Description

    Rat monoclonal [H35-17.2] to CD8 beta (FITC)
  • Host species

    Rat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c

  • Positive control

    • BALB/c Mouse splenocytes

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    H35-17.2
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125-0.25µg for 105-8 cells.

A final volume of 100 µL is recommended for staining the cell sample.

 

 

 

ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
  • Tissue specificity

    Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Phosphorylated as a consequence of T-cell activation.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD8b antigen antibody
    • CD8 antigen beta polypeptide 1 (p37) antibody
    • CD8 antigen beta polypeptide 1 antibody
    • CD8 beta antibody
    • CD8b antibody
    • CD8b molecule antibody
    • CD8B_HUMAN antibody
    • CD8B1 antibody
    • Leu2 antibody
    • Ly3 antibody
    • LYT3 antibody
    • MGC119115 antibody
    • P37 antibody
    • T cell surface glycoprotein CD8 beta chain antibody
    • T lymphocyte surface glycoprotein beta chain antibody
    • T-cell surface glycoprotein CD8 beta chain antibody
    see all

Images

  • Staining of BALB/c splenocytes with Anti-Mouse CD8a APC and 0.125 µg of Rat IgG2b ? Isotype Control FITC (left) or ab95773 (right).
    Cells in the lymphocyte gate were used for analysis.

References

This product has been referenced in:

  • Thoma-Uszynski S  et al. CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice. Microb Pathog 23:101-6 (1997). Read more (PubMed: 9245621) »
  • Tarleton RL  et al. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun 62:1820-9 (1994). Read more (PubMed: 8168945) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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