Overview

  • Product name

    Anti-CD8 beta antibody [H35-17.2] (Phycoerythrin)
    See all CD8 beta primary antibodies
  • Description

    Rat monoclonal [H35-17.2] to CD8 beta (Phycoerythrin)
  • Host species

    Rat
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    5-day mixed leukocyte culture population, C57BL/6 anti-BALB/c

  • Positive control

    • Mouse splenocytes.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: less than or equal to 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    H35-17.2
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.06-0.125µg for 105-8 cells.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
  • Tissue specificity

    Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Phosphorylated as a consequence of T-cell activation.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD8b antigen antibody
    • CD8 antigen beta polypeptide 1 (p37) antibody
    • CD8 antigen beta polypeptide 1 antibody
    • CD8 beta antibody
    • CD8b antibody
    • CD8b molecule antibody
    • CD8B_HUMAN antibody
    • CD8B1 antibody
    • Leu2 antibody
    • Ly3 antibody
    • LYT3 antibody
    • MGC119115 antibody
    • P37 antibody
    • T cell surface glycoprotein CD8 beta chain antibody
    • T lymphocyte surface glycoprotein beta chain antibody
    • T-cell surface glycoprotein CD8 beta chain antibody
    see all

Images

  • Staining of C57BL/6 splenocytes with Anti-Mouse CD8a FITC and 0.06 µg of Rat IgG2b ? Isotype Control PE (left) or ab95772 (right) by Flow Cytometry. Cells in the lymphocyte gate were used for analysis.

References

ab95772 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab95772.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up