Anti-CD80 antibody [16-10A1] - Low endotoxin, Azide free (ab171220)
![Flow Cytometry - Anti-CD80 antibody [16-10A1] - Low endotoxin, Azide free (ab171220)](https://www.abcam.com/ps/products/171/ab171220/Images/ab171220-391496-anti-cd80-antibody-tonbo-flow-cyt.png "Flow Cytometry - Anti-CD80 antibody [16-10A1] - Low endotoxin, Azide free (ab171220)")
Key features and details
- Armenian hamster monoclonal [16-10A1] to CD80 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-CD80 antibody [16-10A1] - Low endotoxin, Azide free
See all CD80 primary antibodies -
Description
Armenian hamster monoclonal [16-10A1] to CD80 - Low endotoxin, Azide free -
Host species
Armenian hamster -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- C57BI/6 splenocytes stimulated with anti-IgM and anti-CD40.
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General notes
Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
16-10A1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12519 Mouse
- SwissProt: Q00609 Mouse
- Unigene: 89474 Mouse
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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Flow Cytometry - Anti-CD80 antibody [16-10A1] - Low endotoxin, Azide free (ab171220)
This data was developed using the same antibody clone in a different buffer formation (ab106162).
Flow cytometric analysis of C57BI/6 splenocytes stimulated with anti-IgM and anti-CD40 for 4 days. Cells were stained with 0.06 µg ab106162 (blue line) or 0.06 µg APC Armenian Hamster isotype control (dashed line).
Datasheets and documents
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Datasheet download
References (0)
ab171220 has not yet been referenced specifically in any publications.
