Key features and details
- Rabbit polyclonal to CD80
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab215166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Tissue specificityExpressed on activated B-cells, macrophages and dendritic cells.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
Anti-CD80 antibody (ab215166) at 1/500 dilution + Mouse spleen lysates
Conjugated secondary antibody at 1/10000 dilution
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat brain tissue labeling CD80 with ab215166 at 1/400 dilution overnight followed by a conjugated secondary and DAB staining.
ab215166 has been referenced in 1 publication.
- Peng KT et al. Particulate matter exposure aggravates osteoarthritis severity. Clin Sci (Lond) 133:2171-2187 (2019). PubMed: 31696218