Anti-CD80 antibody (ab254579)
Key features and details
- Rabbit polyclonal to CD80
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD80 antibody
See all CD80 primary antibodies -
Description
Rabbit polyclonal to CD80 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human CD80 aa 60-110. The exact sequence is proprietary. NP_005182. 17 amino acids long.
Database link: P33681 -
Positive control
- WB: EL4 cell lysate. IF: Human tonsil tissue. IHC-P: Human tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa.
WB is only validated using mouse samples. |
Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa. WB is only validated using mouse samples. |
Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 941 Human
- Entrez Gene: 12519 Mouse
- Omim: 112203 Human
- SwissProt: P33681 Human
- SwissProt: Q00609 Mouse
- Unigene: 838 Human
- Unigene: 89474 Mouse
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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Human tonsil tissue stained for CD80 with ab254579 at 2 µg/ml in immunohistochemical analysis.
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Human tonsil tissue labeling CD80 using ab254579 at 20 µg/ml in IF.
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All lanes : Anti-CD80 antibody (ab254579) at 1 µg/ml
Lane 1 : EL4 (Mouse cell line) cell lysate
Lane 2 : EL4 (Mouse cell line) cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 33 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab254579 has been referenced in 2 publications.
- Zhou X et al. In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis. Nat Commun 13:5700 (2022). PubMed: 36171212
- Alsheikh HAM et al. Normalizing glucose levels reconfigures the mammary tumor immune and metabolic microenvironment and decreases metastatic seeding. Cancer Lett 517:24-34 (2021). PubMed: 34052331