Recombinant
RabMAb

Recombinant Anti-CD80 antibody [EPR1157(2)] (ab134120)

Overview

  • Product name

    Anti-CD80 antibody [EPR1157(2)]
    See all CD80 primary antibodies
  • Description

    Rabbit monoclonal [EPR1157(2)] to CD80
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD80 aa 250 to the C-terminus.

  • Positive control

    • WB: Raji and Ramos cell lysates. IHC-P: human tonsil tissue FC: Raji cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa).

For unpurified use at 1/500.

IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
    • Tissue specificity

      Expressed on activated B-cells, macrophages and dendritic cells.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Activation B7-1 antigen antibody
      • B lymphocyte activation antigen B7 antibody
      • B7 antibody
      • B7-1 antibody
      • B7-1 antigen antibody
      • B7.1 antibody
      • BB1 antibody
      • CD28 antigen ligand 1 antibody
      • CD28LG antibody
      • CD28LG1 antibody
      • CD80 antibody
      • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
      • CD80 antigen antibody
      • CD80 molecule antibody
      • CD80_HUMAN antibody
      • Costimulatory factor CD80 antibody
      • costimulatory molecule variant IgV-CD80 antibody
      • CTLA-4 counter-receptor B7.1 antibody
      • LAB7 antibody
      • T-lymphocyte activation antigen CD80 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD80 with ab134120 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Trs/EDTA buffer, pH9 (ab93684). ImmunoHistoProbe one step HRP Polymer was used. Counter stained with hematoxylin.

      The image shows cytoplasmic and membrane staining.

    • Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD80 with purified ab134120 at 1:190 dilution (10.33 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
    • Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/2500 dilution (purified) + Raji cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 33 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (unpurified)

      Lane 1 : Raji cell lysate
      Lane 2 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Chen J  et al. Regulatory T cells with a defect in inhibition on co-stimulation deteriorated primary biliary cholangitis. Oncotarget 8:108406-108417 (2017). Read more (PubMed: 29312539) »
    • Ludwig S  et al. Suppression of Lymphocyte Functions by Plasma Exosomes Correlates with Disease Activity in Patients with Head and Neck Cancer. Clin Cancer Res 23:4843-4854 (2017). Read more (PubMed: 28400428) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF

    Abcam user community

    Verified customer

    Submitted May 16 2019

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    tumor
    Fixative
    Formaldehyde

    Miss. Sylvie Colin

    Verified customer

    Submitted Oct 20 2017

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