Recombinant Anti-CD80 antibody [EPR1157(2)] (ab134120)
- Datasheet
- References (8)
- Protocols
Overview
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Product name
Anti-CD80 antibody [EPR1157(2)]
See all CD80 primary antibodies -
Description
Rabbit monoclonal [EPR1157(2)] to CD80 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD80 aa 250 to the C-terminus.
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Positive control
- WB: Raji and Ramos cell lysates. IHC-P: human tonsil tissue FC: Raji cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1157(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa). For unpurified use at 1/500. |
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| IHC-P | 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-269698-anti-cd80-antibody-epr11572-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD80 with ab134120 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Trs/EDTA buffer, pH9 (ab93684). ImmunoHistoProbe one step HRP Polymer was used. Counter stained with hematoxylin.
The image shows cytoplasmic and membrane staining.
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![Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-333814-anti-cd80-antibody-epr11572-flow-cytometry.jpg)
Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD80 with purified ab134120 at 1:190 dilution (10.33 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green -
![Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-239863-anti-cd80-antibody-epr11572-western-blot.jpg)
Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/2500 dilution (purified) + Raji cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-148331-anti-cd80-antibody-epr11572-western-blot.jpg)
Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (unpurified)
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
References
This product has been referenced in:
- Amponsa VO et al. Carcinogen-induced bladder cancer in the FVB mouse strain is associated with glandular differentiation and increased Cd274/Pdl-1 expression. Am J Clin Exp Urol 7:139-152 (2019). Read more (PubMed: 31317053) »
- Gunasekaran M et al. Circulating Exosomes with Distinct Properties during Chronic Lung Allograft Rejection. J Immunol 200:2535-2541 (2018). Read more (PubMed: 29491008) »
