Recombinant Anti-CD80 antibody [EPR1157(2)] (ab134120)
![Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-333814-anti-cd80-antibody-epr11572-flow-cytometry.jpg "Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1157(2)] to CD80
- Suitable for: Flow Cyt, WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD80 antibody [EPR1157(2)]
See all CD80 primary antibodies -
Description
Rabbit monoclonal [EPR1157(2)] to CD80 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji and Ramos cell lysates. IHC-P: human tonsil Tissue Flow Cyt: Raji cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1157(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa).
For unpurified use at 1/500. |
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| IHC-P | (2) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa). For unpurified use at 1/500. |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD80 with purified ab134120 at 1:190 dilution (10.33 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-269698-anti-cd80-antibody-epr11572-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD80 with ab134120 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Trs/EDTA buffer, pH9 (ab93684). ImmunoHistoProbe one step HRP Polymer was used. Counter stained with hematoxylin.
The image shows cytoplasmic and membrane staining.
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![Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-415035-anti-cd80-antibody-epr11572-western-blot-raji-wt-raji-cd80-knockout-ramos.jpg)
Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution
Lane 1 : Wild-type Raji cell lysate
Lane 2 : CD80 knockout Raji cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab134120 observed at 55 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab134120 was shown to react with CD80 in wild-type Raji cells in western blot with loss of signal observed in CD80 knockout sample. Wild-type and CD80 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134120 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-239863-anti-cd80-antibody-epr11572-western-blot.jpg)
Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/2500 dilution (purified) + Raji cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-148331-anti-cd80-antibody-epr11572-western-blot.jpg)
Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (unpurified)
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-10-anti-cd80-antibody-epr1157-2-tissue-microarray-human-ab134120.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)Tissue Microarrays stained for "Anti-CD80 antibody [EPR1157(2)]” using "ab134120" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab134120 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.com/ps/products/134/ab134120/Images/ab134120-8-benefits-of-recombinant-antibodies.png)
Anti-CD80 antibody [EPR1157(2)] (ab134120)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab134120 has been referenced in 15 publications.
- Zhang L et al. Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer. Cell 181:442-459.e29 (2020). PubMed: 32302573
- Kuwabara S et al. Prognostic relevance of tertiary lymphoid organs following neoadjuvant chemoradiotherapy in pancreatic ductal adenocarcinoma. Cancer Sci 110:1853-1862 (2019). PubMed: 30997706
- Cui CY et al. Skewed macrophage polarization in aging skeletal muscle. Aging Cell 18:e13032 (2019). PubMed: 31478346
- Nascimento Da Conceicao V et al. Loss of Ca2+ entry via Orai-TRPC1 induces ER stress, initiating immune activation in macrophages. J Cell Sci 133:N/A (2019). PubMed: 31722977
- Jaiswal SR et al. CTLA4Ig-based reduced intensity conditioning and donor lymphocyte infusions for haploidentical transplantation in refractory aggressive B-cell lymphoma relapsing after an autograft: Early results from a pilot study. Exp Hematol 77:26-35.e1 (2019). PubMed: 31449833