For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cd80-antibody-epr11572-low-endotoxin-azide-free-ab224797.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity B Cells CD
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
  • Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
  • Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
  • Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
  • Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
  • Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1157(2)] to CD80 - Low endotoxin, Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Conjugation
Product image
PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
Protein
Product image
Recombinant Human CD80 protein (ab131745)

View more associated products

Overview

  • Product name

    Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free
    See all CD80 primary antibodies
  • Description

    Rabbit monoclonal [EPR1157(2)] to CD80 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD80 aa 250 to the C-terminus.

  • Positive control

    • WB: Raji and Ramos cell lysates. IHC-P: Human tonsil tissue. Flow Cyt: Raji cells.
  • General notes

    ab224797 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1157(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Associated products

  • Alternative Versions

    • Anti-CD80 antibody [EPR1157(2)] (ab134120)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji membrane extract lysate (ab30126)
    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant Human CD80 protein (ab131745)

Applications

Our Abpromise guarantee covers the use of ab224797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
    • Tissue specificity

      Expressed on activated B-cells, macrophages and dendritic cells.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P33681 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 941 Human
      • Omim: 112203 Human
      • SwissProt: P33681 Human
      • Unigene: 838 Human
      • Alternative names

        • Activation B7-1 antigen antibody
        • B lymphocyte activation antigen B7 antibody
        • B7 antibody
        • B7-1 antibody
        • B7-1 antigen antibody
        • B7.1 antibody
        • BB1 antibody
        • CD28 antigen ligand 1 antibody
        • CD28LG antibody
        • CD28LG1 antibody
        • CD80 antibody
        • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
        • CD80 antigen antibody
        • CD80 molecule antibody
        • CD80_HUMAN antibody
        • Costimulatory factor CD80 antibody
        • costimulatory molecule variant IgV-CD80 antibody
        • CTLA-4 counter-receptor B7.1 antibody
        • LAB7 antibody
        • T-lymphocyte activation antigen CD80 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)

        This IHC data was generated using the same anti-CD80 antibody clone [EPR1157(2)] in a different buffer formulation (cat# ab134120).

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD80 with ab134120 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Trs/EDTA buffer, pH9 (ab93684). ImmunoHistoProbe one step HRP Polymer was used. Counter stained with hematoxylin.

        The image shows cytoplasmic and membrane staining.

      • Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)

        Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD80 with purified ab134120 at 1:190 dilution (10.33 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224797)

      • Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution

        Lane 1 : Wild-type Raji cell lysate
        Lane 2 : CD80 knockout Raji cell lysate
        Lane 3 : Ramos cell lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 33 kDa
        Observed band size: 55 kDa
        why is the actual band size different from the predicted?



        This data was developed using the same antibody clone in a different buffer formulation (ab134120).

        Lanes 1 - 3: Merged signal (red and green). Green - ab134120 observed at 55 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

        ab134120 was shown to react with CD80 in wild-type Raji cells in western blot with loss of signal observed in CD80 knockout sample. Wild-type and CD80 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134120 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (Unpurified)

        Lane 1 : Raji cell lysate
        Lane 2 : Ramos cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

        Predicted band size: 33 kDa
        Observed band size: 60 kDa why is the actual band size different from the predicted?



        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224797)

      • Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        Western blot - Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (unpurified)

        Lane 1 : Raji cell lysate
        Lane 2 : Ramos cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

        Predicted band size: 33 kDa
        Observed band size: 60 kDa why is the actual band size different from the predicted?



        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224797)

      • Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)
        Anti-CD80 antibody [EPR1157(2)] - Low endotoxin, Azide free (ab224797)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (3)

      Publishing research using ab224797? Please let us know so that we can cite the reference in this datasheet.

      ab224797 has been referenced in 3 publications.

      • Müller A  et al. Towards non-invasive imaging of vulnerable atherosclerotic plaques by targeting co-stimulatory molecules. Int J Cardiol 174:503-15 (2014). IHC ; Human . PubMed: 24834996
      • Kolyva AS  et al. The role of obesity in the immune response during sepsis. Nutr Diabetes 4:e137 (2014). IHC-P ; Human . PubMed: 25244356
      • Jia R  et al. The splicing factor hnRNP C regulates expression of co-stimulatory molecules CD80 and CD40 in dendritic cells. Immunol Lett 153:27-32 (2013). PubMed: 23831410

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab224797.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.