Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22127-251] to CD80 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
Product nameAnti-CD80 antibody [EPR22127-251] - BSA and Azide free
See all CD80 primary antibodies
DescriptionRabbit monoclonal [EPR22127-251] to CD80 - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD80 aa 1-250. The exact sequence is proprietary.
Database link: Q00609
- Flow cyt: DC2.4 cells.
ab238649 is the carrier-free version of ab222702. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab238649 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab238649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Tissue specificityExpressed on activated B-cells, macrophages and dendritic cells.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
Flow cytometric analysis of P815 (mouse mastocytoma mast cell line)(left panel) cells and DC2.4 (mouse dentric cell line)(right panel) cells labeling CD80 with ab222702 at 1/500 (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: P815 (PMID: 9237108).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222702).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238649 has not yet been referenced specifically in any publications.