Recombinant
RabMAb

Recombinant Anti-CD80 antibody [EPR22127-317] - BSA and Azide free (ab238650)

Overview

  • Product name

    Anti-CD80 antibody [EPR22127-317] - BSA and Azide free
    See all CD80 primary antibodies
  • Description

    Rabbit monoclonal [EPR22127-317] to CD80 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse CD80 aa 1-250. The exact sequence is proprietary.
    Database link: Q00609

  • Positive control

    • WB: C2C12 and DC2.4 whole cell lysates.
  • General notes

    Ab238650 is the carrier-free version of ab238481. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238650 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 55-60 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificity

    Expressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation B7-1 antigen antibody
    • B lymphocyte activation antigen B7 antibody
    • B7 antibody
    • B7-1 antibody
    • B7-1 antigen antibody
    • B7.1 antibody
    • BB1 antibody
    • CD28 antigen ligand 1 antibody
    • CD28LG antibody
    • CD28LG1 antibody
    • CD80 antibody
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
    • CD80 antigen antibody
    • CD80 molecule antibody
    • CD80_HUMAN antibody
    • Costimulatory factor CD80 antibody
    • costimulatory molecule variant IgV-CD80 antibody
    • CTLA-4 counter-receptor B7.1 antibody
    • LAB7 antibody
    • T-lymphocyte activation antigen CD80 antibody
    see all

Images

  • All lanes : Anti-CD80 antibody [EPR22127-317] (ab238481) at 1/1000 dilution

    Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 2 : DC2.4 (mouse dendritic cell line) whole cell lysate
    Lane 3 : P815 (mouse mastocytoma mast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa
    Observed band size: 55-60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 7544014, PMID: 9079798); 55-60 kDa is due to glycosylation (PMID: 9654121).

    Negative control: P815 (PMID: 9237108).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238481).

References

ab238650 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab238650.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up