Product nameAnti-CD80 antibody [EPR22127-317] - BSA and Azide free
See all CD80 primary antibodies
DescriptionRabbit monoclonal [EPR22127-317] to CD80 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD80 aa 1-250. The exact sequence is proprietary.
Database link: Q00609
- WB: C2C12 and DC2.4 whole cell lysates.
Ab238650 is the carrier-free version of ab238481. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab238650 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab238650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 55-60 kDa (predicted molecular weight: 35 kDa).|
FunctionInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Tissue specificityExpressed on activated B-cells, macrophages and dendritic cells.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
All lanes : Anti-CD80 antibody [EPR22127-317] (ab238481) at 1/1000 dilution
Lane 1 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 2 : DC2.4 (mouse dendritic cell line) whole cell lysate
Lane 3 : P815 (mouse mastocytoma mast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?
Exposure time: 26 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 7544014, PMID: 9079798); 55-60 kDa is due to glycosylation (PMID: 9654121).
Negative control: P815 (PMID: 9237108).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238481).
ab238650 has not yet been referenced specifically in any publications.