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Immunology Adaptive Immunity B Cells CD
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RecombinantRabMAb

Recombinant Anti-CD80 antibody [EPR22183] (ab225674)

  • Datasheet
  • Certificate of Compliance
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Abpromise

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Find out more.

Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)
  • Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)
  • Anti-CD80 antibody [EPR22183] (ab225674)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22183] to CD80
  • Suitable for: ICC/IF, Flow Cyt, IP
  • Reacts with: Human

You may also be interested in

Protein
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Recombinant Human CD80 protein (ab131745)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
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Anti-CD163 antibody [EPR19518] (ab182422)

View more associated products

Overview

  • Product name

    Anti-CD80 antibody [EPR22183]
    See all CD80 primary antibodies
  • Description

    Rabbit monoclonal [EPR22183] to CD80
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Raji cells. IP: Raji whole cell lysate. Flow cyt: Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22183
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Associated products

  • Alternative Versions

    • Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD80 protein (ab131745)
  • Related Products

    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IP
Human
Application Abreviews Notes
ICC/IF
1/1000.
Flow Cyt
1/400.
IP
1/30.
Notes
ICC/IF
1/1000.
Flow Cyt
1/400.
IP
1/30.

Target

  • Function

    Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificity

    Expressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P33681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 941 Human
    • Omim: 112203 Human
    • SwissProt: P33681 Human
    • Unigene: 838 Human
    • Alternative names

      • Activation B7-1 antigen antibody
      • B lymphocyte activation antigen B7 antibody
      • B7 antibody
      • B7-1 antibody
      • B7-1 antigen antibody
      • B7.1 antibody
      • BB1 antibody
      • CD28 antigen ligand 1 antibody
      • CD28LG antibody
      • CD28LG1 antibody
      • CD80 antibody
      • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
      • CD80 antigen antibody
      • CD80 molecule antibody
      • CD80_HUMAN antibody
      • Costimulatory factor CD80 antibody
      • costimulatory molecule variant IgV-CD80 antibody
      • CTLA-4 counter-receptor B7.1 antibody
      • LAB7 antibody
      • T-lymphocyte activation antigen CD80 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)
      Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)

      Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell line) (left panel) cells and Raji (human Burkitt's lymphoma cell line)(right panel) cells labeling CD80  with ab225674 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

      Negative control: HUVEC (PMID: 15064090).

    • Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)
      Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)

      CD80 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab225674 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225674 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Raji whole cell lysate 10 μg (Input).
      Lane 2: ab225674 IP in Raji whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225674 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 1 second.

      This blot was developed using a higher sensitivity ECL substrate.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)
      Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)

      Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) and HUVEC (human umbilical vein endothelial cell line) cells labeling CD80 with ab225674 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      PBS only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution dilution.

      Negative control: HUVEC (PMID: 15064090).

    • Anti-CD80 antibody [EPR22183] (ab225674)
      Anti-CD80 antibody [EPR22183] (ab225674)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab225674? Please let us know so that we can cite the reference in this datasheet.

    ab225674 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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