Recombinant
RabMAb

Recombinant Anti-CD80 antibody [EPR22183] (ab225674)

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Overview

  • Product name

    Anti-CD80 antibody [EPR22183]
    See all CD80 primary antibodies

  • Description

    Rabbit monoclonal [EPR22183] to CD80

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human CD80 aa 1-250. The exact sequence is proprietary.
    Database link: P33681

  • Positive control

    • ICC/IF: Raji cells. IP: Raji whole cell lysate. Flow cyt: Raji cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab225674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
Flow Cyt 1/400.
IP 1/30.

Target

  • Function

    Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

  • Tissue specificity

    Expressed on activated B-cells, macrophages and dendritic cells.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activation B7-1 antigen antibody
      • B lymphocyte activation antigen B7 antibody
      • B7 antibody
      • B7-1 antibody
      • B7-1 antigen antibody
      • B7.1 antibody
      • BB1 antibody
      • CD28 antigen ligand 1 antibody
      • CD28LG antibody
      • CD28LG1 antibody
      • CD80 antibody
      • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
      • CD80 antigen antibody
      • CD80 molecule antibody
      • CD80_HUMAN antibody
      • Costimulatory factor CD80 antibody
      • costimulatory molecule variant IgV-CD80 antibody
      • CTLA-4 counter-receptor B7.1 antibody
      • LAB7 antibody
      • T-lymphocyte activation antigen CD80 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)
      Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)

      Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell line) (left panel) cells and Raji (human Burkitt's lymphoma cell line)(right panel) cells labeling CD80  with ab225674 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

      Negative control: HUVEC (PMID: 15064090).

    • Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)
      Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)

      CD80 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab225674 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225674 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Raji whole cell lysate 10 μg (Input).
      Lane 2: ab225674 IP in Raji whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225674 in Raji whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 1 second.

      This blot was developed using a higher sensitivity ECL substrate.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)
      Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)

      Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) and HUVEC (human umbilical vein endothelial cell line) cells labeling CD80 with ab225674 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      PBS only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution dilution.

      Negative control: HUVEC (PMID: 15064090).

    References

    ab225674 has not yet been referenced specifically in any publications.

    Publishing research using ab225674? Please let us know so that we can cite the reference in this datasheet.

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