Recombinant Anti-CD80 antibody [EPR22183] (ab225674)
![Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)](https://www.abcam.com/ps/products/225/ab225674/Images/ab225674-316585-anti-cd80-antibody-epr22183-flow-cytometry.jpg "Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22183] to CD80
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-CD80 antibody [EPR22183]
See all CD80 primary antibodies -
Description
Rabbit monoclonal [EPR22183] to CD80 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Raji cells. IP: Raji whole cell lysate. Flow cyt: Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22183 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IP |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000.
|
|
| Flow Cyt |
1/400.
|
|
| IP |
1/30.
|
| Notes |
|---|
|
ICC/IF
1/1000. |
|
Flow Cyt
1/400. |
|
IP
1/30. |
Target
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Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
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Alternative names
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
Images
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Flow Cytometry - Anti-CD80 antibody [EPR22183] (ab225674)
Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell line) (left panel) cells and Raji (human Burkitt's lymphoma cell line)(right panel) cells labeling CD80 with ab225674 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: HUVEC (PMID: 15064090).
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![Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)](https://www.abcam.com/ps/products/225/ab225674/Images/ab225674-316586-anti-cd80-antibody-epr22183-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD80 antibody [EPR22183] (ab225674)CD80 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab225674 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225674 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Raji whole cell lysate 10 μg (Input).
Lane 2: ab225674 IP in Raji whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225674 in Raji whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.This blot was developed using a higher sensitivity ECL substrate.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)](https://www.abcam.com/ps/products/225/ab225674/Images/ab225674-316584-anti-cd80-antibody-epr22183-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD80 antibody [EPR22183] (ab225674)Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) and HUVEC (human umbilical vein endothelial cell line) cells labeling CD80 with ab225674 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
PBS only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution dilution.Negative control: HUVEC (PMID: 15064090).
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![Anti-CD80 antibody [EPR22183] (ab225674)](https://www.abcam.com/ps/products/225/ab225674/Images/ab225674-4-benefits-of-recombinant-antibodies.png)
Anti-CD80 antibody [EPR22183] (ab225674)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab225674 has not yet been referenced specifically in any publications.
