Recombinant
RabMAb

Recombinant Anti-CD80 antibody [EPR22183] - BSA and Azide free (ab238648)

Overview

  • Product name

    Anti-CD80 antibody [EPR22183] - BSA and Azide free
    See all CD80 primary antibodies
  • Description

    Rabbit monoclonal [EPR22183] to CD80 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD80 aa 1-250. The exact sequence is proprietary.
    Database link: P33681

  • Positive control

    • ICC/IF: Raji cells.
  • General notes

    Ab238648 is the carrier-free version of ab225674. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238648 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
  • Tissue specificity

    Expressed on activated B-cells, macrophages and dendritic cells.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation B7-1 antigen antibody
    • B lymphocyte activation antigen B7 antibody
    • B7 antibody
    • B7-1 antibody
    • B7-1 antigen antibody
    • B7.1 antibody
    • BB1 antibody
    • CD28 antigen ligand 1 antibody
    • CD28LG antibody
    • CD28LG1 antibody
    • CD80 antibody
    • CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) antibody
    • CD80 antigen antibody
    • CD80 molecule antibody
    • CD80_HUMAN antibody
    • Costimulatory factor CD80 antibody
    • costimulatory molecule variant IgV-CD80 antibody
    • CTLA-4 counter-receptor B7.1 antibody
    • LAB7 antibody
    • T-lymphocyte activation antigen CD80 antibody
    see all

Images

  • Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell line) (left panel) cells and Raji (human Burkitt's lymphoma cell line)(right panel) cells labeling CD80  with ab225674 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: HUVEC (PMID: 15064090).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225674).

  • CD80 was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab225674 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab225674 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Raji whole cell lysate 10 μg (Input).
    Lane 2: ab225674 IP in Raji whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab225674 in Raji whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    This blot was developed using a higher sensitivity ECL substrate.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225674).

  • Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) and HUVEC (human umbilical vein endothelial cell line) cells labeling CD80 with ab225674 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    PBS only control: Used PBS instead of primary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: HUVEC cells (PMID: 15064090).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225674).

References

ab238648 has not yet been referenced specifically in any publications.

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