Anti-CD81 antibody [1D6] (ab23505)
![Flow Cytometry - Anti-CD81 antibody [1D6] (ab23505)](https://www.abcam.com/ps/products/23/ab23505/Images/ab23505-308624-anti-cd81-antibody-1d6-flow-cytometry.jpg "Flow Cytometry - Anti-CD81 antibody [1D6] (ab23505)")
Key features and details
- Mouse monoclonal [1D6] to CD81
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD81 antibody [1D6]
See all CD81 primary antibodies -
Description
Mouse monoclonal [1D6] to CD81 -
Host species
Mouse -
Specificity
ab23505 recognises human CD81, a 26kD cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Goat, Chimpanzee
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Immunogen
Tissue, cells or virus corresponding to Human CD81. Aggregated OCI-LY8 cells (Human).
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Positive control
- Flow Cyt: HAP1-WT cells.
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General notes
This antibody induces homotypic adhesion and has powerful anti proliferative effects.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
This antibody induces homotypic adhesion and has powerful anti proliferative effects. -
Clonality
Monoclonal -
Clone number
1D6 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/5 - 1/10.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
Flow Cyt
1/5 - 1/10. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
Tissue specificity
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
Involvement in disease
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsNot glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 449635 Chimpanzee
- Entrez Gene: 975 Human
- Omim: 186845 Human
- SwissProt: P60034 Chimpanzee
- SwissProt: P60033 Human
- Unigene: 54457 Human
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Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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Flow Cytometry - Anti-CD81 antibody [1D6] (ab23505)
Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab23505. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab23505, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.
A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab23505 has been referenced in 10 publications.
- Sayeed A et al. Profiling the circulating mRNA transcriptome in human liver disease. Oncotarget 11:2216-2232 (2020). PubMed: 32577166
- Krishn SR et al. The avß6 integrin in cancer cell-derived small extracellular vesicles enhances angiogenesis. J Extracell Vesicles 9:1763594 (2020). PubMed: 32595914
- DeRita RM et al. Tumor-Derived Extracellular Vesicles Require ß1 Integrins to Promote Anchorage-Independent Growth. iScience 14:199-209 (2019). PubMed: 30981115
- Lu H et al. Exosomal avß6 integrin is required for monocyte M2 polarization in prostate cancer. Matrix Biol 70:20-35 (2018). WB ; Human . PubMed: 29530483
- Desplantes R et al. Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor. Sci Rep 7:1032 (2017). PubMed: 28432329
- DeRita RM et al. c-Src, Insulin-Like Growth Factor I Receptor, G-Protein-Coupled Receptor Kinases and Focal Adhesion Kinase are Enriched Into Prostate Cancer Cell Exosomes. J Cell Biochem 118:66-73 (2017). PubMed: 27232975
- Manzoni G et al. Plasmodium P36 determines host cell receptor usage during sporozoite invasion. Elife 6:N/A (2017). PubMed: 28506360
- Trerotola M et al. Trop-2 is up-regulated in invasive prostate cancer and displaces FAK from focal contacts. Oncotarget 6:14318-28 (2015). PubMed: 26015409
- Christianson HC et al. Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity. Proc Natl Acad Sci U S A 110:17380-5 (2013). WB ; Human . PubMed: 24101524
- Wang AL et al. Autophagy and exosomes in the aged retinal pigment epithelium: possible relevance to drusen formation and age-related macular degeneration. PLoS One 4:e4160 (2009). Flow Cyt ; Human . PubMed: 19129916