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    cd81-antibody-1d6-ab23505.pdf

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Immunology Adaptive Immunity B Cells CD
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Validated using a knockout cell line

Anti-CD81 antibody [1D6] (ab23505)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (10)

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Flow Cytometry - Anti-CD81 antibody [1D6] (ab23505)

    Key features and details

    • Mouse monoclonal [1D6] to CD81
    • Suitable for: Flow Cyt
    • Knockout validated
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-CD81 antibody [1D6]
      See all CD81 primary antibodies
    • Description

      Mouse monoclonal [1D6] to CD81
    • Host species

      Mouse
    • Specificity

      ab23505 recognises human CD81, a 26kD cell surface antigen also known as TAPA-1, and a member of the tetraspanin family.
    • Tested applications

      Suitable for: Flow Cytmore details
      Unsuitable for: WB
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Sheep, Goat, Chimpanzee
    • Immunogen

      Tissue, cells or virus corresponding to Human CD81. Aggregated OCI-LY8 cells (Human).

    • Positive control

      • Flow Cyt: HAP1-WT cells.
    • General notes


      This antibody induces homotypic adhesion and has powerful anti proliferative effects.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Primary antibody notes

      This antibody induces homotypic adhesion and has powerful anti proliferative effects.
    • Clonality

      Monoclonal
    • Clone number

      1D6
    • Myeloma

      P3x63-Ag8.653
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Microbiology
      • Protein
      • Human Protein
      • Receptor

    Associated products

    • Alternative Versions

      • FITC Anti-CD81 antibody [1D6] (ab33139)
      • Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human CD81 protein (ab152267)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab23505 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    1/5 - 1/10.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    1/5 - 1/10.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
    • Tissue specificity

      Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
    • Involvement in disease

      Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.
    • Post-translational
      modifications

      Not glycosylated.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P60033 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 449635 Chimpanzee
      • Entrez Gene: 975 Human
      • Omim: 186845 Human
      • SwissProt: P60034 Chimpanzee
      • SwissProt: P60033 Human
      • Unigene: 54457 Human
      • Alternative names

        • 26 kDa cell surface protein TAPA 1 antibody
        • 26 kDa cell surface protein TAPA-1 antibody
        • 26 kDa cell surface protein TAPA1 antibody
        • CD 81 antibody
        • CD81 antibody
        • CD81 antigen (target of antiproliferative antibody 1) antibody
        • CD81 antigen antibody
        • CD81 molecule antibody
        • CD81_HUMAN antibody
        • CVID6 antibody
        • S5.7 antibody
        • TAPA 1 antibody
        • TAPA1 antibody
        • Target of the antiproliferative antibody 1 antibody
        • Tetraspanin 28 antibody
        • Tetraspanin-28 antibody
        • Tetraspanin28 antibody
        • Tspan 28 antibody
        • Tspan-28 antibody
        • Tspan28 antibody
        see all

      Images

      • Flow Cytometry - Anti-CD81 antibody [1D6] (ab23505)
        Flow Cytometry - Anti-CD81 antibody [1D6] (ab23505)

        Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab23505. The cells were fixed with 4% formaldehyde (10 min)  and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab23505, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

        A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

        Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

        This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (10)

      Publishing research using ab23505? Please let us know so that we can cite the reference in this datasheet.

      ab23505 has been referenced in 10 publications.

      • Sayeed A  et al. Profiling the circulating mRNA transcriptome in human liver disease. Oncotarget 11:2216-2232 (2020). PubMed: 32577166
      • Krishn SR  et al. The avß6 integrin in cancer cell-derived small extracellular vesicles enhances angiogenesis. J Extracell Vesicles 9:1763594 (2020). PubMed: 32595914
      • DeRita RM  et al. Tumor-Derived Extracellular Vesicles Require ß1 Integrins to Promote Anchorage-Independent Growth. iScience 14:199-209 (2019). PubMed: 30981115
      • Lu H  et al. Exosomal avß6 integrin is required for monocyte M2 polarization in prostate cancer. Matrix Biol 70:20-35 (2018). WB ; Human . PubMed: 29530483
      • Desplantes R  et al. Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor. Sci Rep 7:1032 (2017). PubMed: 28432329
      • DeRita RM  et al. c-Src, Insulin-Like Growth Factor I Receptor, G-Protein-Coupled Receptor Kinases and Focal Adhesion Kinase are Enriched Into Prostate Cancer Cell Exosomes. J Cell Biochem 118:66-73 (2017). PubMed: 27232975
      • Manzoni G  et al. Plasmodium P36 determines host cell receptor usage during sporozoite invasion. Elife 6:N/A (2017). PubMed: 28506360
      • Trerotola M  et al. Trop-2 is up-regulated in invasive prostate cancer and displaces FAK from focal contacts. Oncotarget 6:14318-28 (2015). PubMed: 26015409
      • Christianson HC  et al. Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity. Proc Natl Acad Sci U S A 110:17380-5 (2013). WB ; Human . PubMed: 24101524
      • Wang AL  et al. Autophagy and exosomes in the aged retinal pigment epithelium: possible relevance to drusen formation and age-related macular degeneration. PLoS One 4:e4160 (2009). Flow Cyt ; Human . PubMed: 19129916

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-TAPA1 antibody [1D6]

      Excellent
      Abreviews
      Abreviews
      This product is known to not work in this application or species.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (gastric)
      Loading amount
      20 µg
      Specification
      gastric
      Gel Running Conditions
      Non-reduced Denaturing (4-12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 19 2009

      Question

      I am writing regarding a purchase of the abcam anti Tapa1 antibody that I purchased in Jan this year. I am quite convinced that the antibody that was sent to me does not seem to work in western blots. We work in the proteomics facility in the Glycobiology institute of the department of biochemistry, Univ of oxford. We have tried to use this antibody on concentrated cell culture media that were in culture with two different cell lines HepaRG cells - a hepatocellular carcinoma cell line and OCM-1 cells-a uveal melanoma cell line. The cells were grown in appropriate media and the spent media (we call it the secretome) was then concentrated 15 fold and total proteins precipitated using acetone TCA , washed and then loaded on 1D gels. Gels were run in duplicate, westerns done on one and on the other gel we cut bands and did a mass spec analysis of all the proteins. While the proteins failed to show positive even after concentrating them we did detect proteins using the mass spec with a theoretical molecular mass of about 25 kDa and pI of 5.0. Considering the cost that we have paid for the antibody I ask of you to either consider a replacement antibody that would react with CD81 or agree to give us a replacement for another antibody of our choice as our lab very frequently buys antibodies from you. Looking forward to hearing from you.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 10 2006

      Answer

      Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am sorry but the expression of Tapa1 is outside of my area of expertese. I would appreciate it if you could explain to me why you are attempting to detect this protein in the "secretome"? According to the relevance section of this target TAPA1 is a cell surface glycoprotein. I would like to recommend that you include a positive control in your western blots so that you can fully determine whether the quality of the antibody has been compromised. We recommend the use of a tonsil lysate. I appreciate your comments and look forward to hearing from you.

      Read More

      Abcam Scientific Support

      Answered on Mar 14 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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