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    cd81-antibody-ab155760.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD81 antibody (ab155760)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (6)

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Western blot - Anti-CD81 antibody (ab155760)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody (ab155760)

Key features and details

  • Rabbit polyclonal to CD81
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CD81 antibody
    See all CD81 primary antibodies
  • Description

    Rabbit polyclonal to CD81
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment within Human CD81 aa 86-236. The exact sequence is proprietary.
    Database link: P60033

  • Positive control

    • K562 and Lung Papillary Adenocarcinoma tissue.
  • General notes

    Keep as concentrated solution.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Microbiology
    • Protein
    • Human Protein
    • Receptor

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human CD81 protein (ab152267)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P (1)
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Function

    May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
  • Tissue specificity

    Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
  • Involvement in disease

    Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications

    Not glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P60033 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 975 Human
    • Entrez Gene: 12520 Mouse
    • Entrez Gene: 25621 Rat
    • Omim: 186845 Human
    • SwissProt: P60033 Human
    • SwissProt: P35762 Mouse
    • SwissProt: Q62745 Rat
    • Unigene: 54457 Human
    • Unigene: 806 Mouse
    • Unigene: 1975 Rat
    see all
  • Alternative names

    • 26 kDa cell surface protein TAPA 1 antibody
    • 26 kDa cell surface protein TAPA-1 antibody
    • 26 kDa cell surface protein TAPA1 antibody
    • CD 81 antibody
    • CD81 antibody
    • CD81 antigen (target of antiproliferative antibody 1) antibody
    • CD81 antigen antibody
    • CD81 molecule antibody
    • CD81_HUMAN antibody
    • CVID6 antibody
    • S5.7 antibody
    • TAPA 1 antibody
    • TAPA1 antibody
    • Target of the antiproliferative antibody 1 antibody
    • Tetraspanin 28 antibody
    • Tetraspanin-28 antibody
    • Tetraspanin28 antibody
    • Tspan 28 antibody
    • Tspan-28 antibody
    • Tspan28 antibody
    see all

Images

  • Western blot - Anti-CD81 antibody (ab155760)
    Western blot - Anti-CD81 antibody (ab155760)
    Anti-CD81 antibody (ab155760) at 1/1000 dilution + K562 whole cell lysate at 30 µg

    Predicted band size: 25 kDa



    12% SDS PAGE.

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody (ab155760)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody (ab155760)

    Immunohistochemical analysis of paraffin-embedded Lung Papillary Adenocarcinoma tissue labeling CD81 with ab155760 at 1/250 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab155760? Please let us know so that we can cite the reference in this datasheet.

ab155760 has been referenced in 6 publications.

  • Hu H  et al. Exosome-Derived miR-486-5p Regulates Cell Cycle, Proliferation and Metastasis in Lung Adenocarcinoma via Targeting NEK2. Front Bioeng Biotechnol 8:259 (2020). PubMed: 32322578
  • Shang A  et al. Exosomal KRAS mutation promotes the formation of tumor-associated neutrophil extracellular traps and causes deterioration of colorectal cancer by inducing IL-8 expression. Cell Commun Signal 18:52 (2020). PubMed: 32228650
  • Chen F  et al. Hypoxia induced changes in miRNAs and their target mRNAs in extracellular vesicles of esophageal squamous cancer cells. Thorac Cancer 11:570-580 (2020). PubMed: 31922357
  • Liu R  et al. DDP-resistant ovarian cancer cells-derived exosomal microRNA-30a-5p reduces the resistance of ovarian cancer cells to DDP. Open Biol 10:190173 (2020). PubMed: 32343928
  • Wang J  et al. Exosome-Mediated Transfer of lncRNA HOTTIP Promotes Cisplatin Resistance in Gastric Cancer Cells by Regulating HMGA1/miR-218 Axis. Onco Targets Ther 12:11325-11338 (2019). PubMed: 31908497
  • Spinelli C  et al. Molecular subtypes and differentiation programmes of glioma stem cells as determinants of extracellular vesicle profiles and endothelial cell-stimulating activities. J Extracell Vesicles 7:1490144 (2018). PubMed: 30034643

Customer reviews and Q&As

Show All Reviews Q&A
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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD81 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (mast cell tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
Permeabilization
No
Specification
mast cell tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 22 2019

Western blot abreview for Anti-TAPA1 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More

Abcam user community

Verified customer

Submitted Nov 13 2015

Western blot abreview for Anti-TAPA1 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More

Abcam user community

Verified customer

Submitted Jan 07 2014

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