Overview

  • Product name
    Anti-CD81 antibody [Eat 2]
    See all CD81 primary antibodies
  • Description
    Armenian hamster monoclonal [Eat 2] to CD81
  • Host species
    Armenian hamster
  • Specificity
    ab33697 recognises mouse and rat CD81. In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ELISA, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to CD81. Murine B cell line (38C13).

  • Epitope
    extracellular loops of TAPA-1
  • Positive control
    • B cells.
  • General notes
    This antibody was raised in Armenian hamster.


    ab33697 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. ab33697 requires the presence of both extracellular loops of TAPA-1 for binding. Not suitable for IHC-P.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    ab33697 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. ab33697 requires the presence of both extracellular loops of TAPA-1 for binding. Not suitable for IHC-P.
  • Clonality
    Monoclonal
  • Clone number
    Eat 2
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100.
IHC-Fr Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 26 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 21600883

Target

  • Function
    May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
  • Tissue specificity
    Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
  • Involvement in disease
    Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities
    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications
    Not glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26 kDa cell surface protein TAPA 1 antibody
    • 26 kDa cell surface protein TAPA-1 antibody
    • 26 kDa cell surface protein TAPA1 antibody
    • CD 81 antibody
    • CD81 antibody
    • CD81 antigen (target of antiproliferative antibody 1) antibody
    • CD81 antigen antibody
    • CD81 molecule antibody
    • CD81_HUMAN antibody
    • CVID6 antibody
    • S5.7 antibody
    • TAPA 1 antibody
    • TAPA1 antibody
    • Target of the antiproliferative antibody 1 antibody
    • Tetraspanin 28 antibody
    • Tetraspanin-28 antibody
    • Tetraspanin28 antibody
    • Tspan 28 antibody
    • Tspan-28 antibody
    • Tspan28 antibody
    see all

References

This product has been referenced in:
  • Hanagata N & Li X Osteoblast-enriched membrane protein IFITM5 regulates the association of CD9 with an FKBP11-CD81-FPRP complex and stimulates expression of interferon-induced genes. Biochem Biophys Res Commun 409:378-84 (2011). WB, ICC/IF ; Rat . Read more (PubMed: 21600883) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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