Recombinant Anti-CD81 antibody [EPR4244] (ab109201)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4244] to CD81
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD81 antibody [EPR4244]
See all CD81 primary antibodies -
Description
Rabbit monoclonal [EPR4244] to CD81 -
Host species
Rabbit -
Specificity
To detect stronger signal in exosome lysate with this antibody, please try protocols of exosome isolation in PMID: 33184263, PMID: 34502537 , PMID: 32121214, PMID: 32375124.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos, JAR, U87-MG, IM-9, peripheral blood mononuclear cell (PBMC), C6, PC-12, C2C12, mouse brain, mouse heart, A549, 293T, HeLa, Huh7, HCT 116, LNCaP, U-87 and RAW264.7 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4244 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (6) |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
For unpurified use at 1/500 - 1/1000. For WB related protocols, please refer to the Protocols section below. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. For unpurified use at 1/500 - 1/1000. For WB related protocols, please refer to the Protocols section below. |
Target
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Function
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
Tissue specificity
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
Involvement in disease
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsNot glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 975 Human
- Entrez Gene: 12520 Mouse
- Entrez Gene: 25621 Rat
- Omim: 186845 Human
- SwissProt: P60033 Human
- SwissProt: P35762 Mouse
- SwissProt: Q62745 Rat
- Unigene: 54457 Human
see all -
Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution
Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with NFDM/TBST
Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysate with NFDM/TBST
Lane 3 : Mouse brain tissue lysate with NFDM/TBST
Lane 4 : Mouse heart tissue lysate with NFDM/TBST
Lane 5 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 6 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate with NFDM/TBST
Lane 7 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 8 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 9 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 10 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 11 : A549 (Human lung carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 12 : 293T (Human embryonic kidney epithelial cell) whole cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 26 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 60 seconds -
All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/700 dilution (unpurified)
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/2000 dilution (purified)
Lane 1 : Ramos cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : Raw264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution (unpurified)
Lane 1 : Ramos cell lysate
Lane 2 : JAR cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : IM-9 cell lysate
Lane 5 : Peripheral blood mononuclear cell (PBMC) cell lysate
Lane 6 : C6 cell lysate
Lane 7 : PC-12 cell lysate
Lane 8 : RAW264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
Predicted band size: 26 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (196)
ab109201 has been referenced in 196 publications.
- Lai C et al. Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis rats. Mol Cell Biochem 478:637-649 (2023). PubMed: 36001206
- Takada Y et al. Tumor Necrosis Factor-α Blunts the Osteogenic Effects of Muscle Cell-Derived Extracellular Vesicles by Affecting Muscle Cells. Calcif Tissue Int 112:377-388 (2023). PubMed: 36576505
- Tu J et al. Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors. Sci Rep 13:750 (2023). PubMed: 36639385
- Li Y et al. Exosomes Secreted from Adipose-Derived Stem Cells Are a Potential Treatment Agent for Immune-Mediated Alopecia. J Immunol Res 2022:7471246 (2022). PubMed: 35155688
- Shou Y et al. Exosomes-derived miR-154-5p attenuates esophageal squamous cell carcinoma progression and angiogenesis by targeting kinesin family member 14. Bioengineered 13:4610-4620 (2022). PubMed: 35156510