Recombinant
RabMAb

Anti-CD81 antibody [EPR4244] (ab109201)

Rabbit recombinant monoclonal CD81 antibody [EPR4244]. Validated in WB and tested in Mouse, Rat, Human. Cited in 7 publication(s). Independently reviewed in 4 review(s).

Overview

  • Product name
    Anti-CD81 antibody [EPR4244]
    See all CD81 primary antibodies
  • Description
    Rabbit monoclonal [EPR4244] to CD81
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD81 aa 150-250. The exact sequence is proprietary.
    Database link: P60033

  • Positive control
    • WB: Ramos, JAR, U87-MG, IM-9, peripheral blood mononuclear cell (PBMC), C6, PC-12, and RAW264.7 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.

For unpurified use at 1/500 - 1/1000.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
    • Tissue specificity
      Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
    • Involvement in disease
      Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similarities
      Belongs to the tetraspanin (TM4SF) family.
    • Post-translational
      modifications
      Not glycosylated.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 26 kDa cell surface protein TAPA 1 antibody
      • 26 kDa cell surface protein TAPA-1 antibody
      • 26 kDa cell surface protein TAPA1 antibody
      • CD 81 antibody
      • CD81 antibody
      • CD81 antigen (target of antiproliferative antibody 1) antibody
      • CD81 antigen antibody
      • CD81 molecule antibody
      • CD81_HUMAN antibody
      • CVID6 antibody
      • S5.7 antibody
      • TAPA 1 antibody
      • TAPA1 antibody
      • Target of the antiproliferative antibody 1 antibody
      • Tetraspanin 28 antibody
      • Tetraspanin-28 antibody
      • Tetraspanin28 antibody
      • Tspan 28 antibody
      • Tspan-28 antibody
      • Tspan28 antibody
      see all

    Images

    • All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/700 dilution (unpurified)

      Lane 1 : Ramos cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : Raw264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 26 kDa
      Observed band size: 20 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/2000 dilution (purified)

      Lane 1 : Ramos cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : Raw264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 26 kDa
      Observed band size: 20 kDa why is the actual band size different from the predicted?

    • All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution (unpurified)

      Lane 1 : Ramos cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : IM-9 cell lysate
      Lane 5 : Peripheral blood mononuclear cell (PBMC) cell lysate
      Lane 6 : C6 cell lysate
      Lane 7 : PC-12 cell lysate
      Lane 8 : RAW264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

      Predicted band size: 26 kDa

    References

    This product has been referenced in:
    • Dong H  et al. Exosome-mediated transfer of lncRNA-SNHG14 promotes trastuzumab chemoresistance in breast cancer. Int J Oncol 53:1013-1026 (2018). Read more (PubMed: 30015837) »
    • Domenis R  et al. Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm 2017:4814987 (2017). WB ; Human . Read more (PubMed: 28634420) »
    See all 7 Publications for this product

    Customer reviews and Q&As

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    1-4 of 4 Abreviews

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (Gradient Gel)
    Sample
    Hamster Cell lysate - whole cell (CHO cells)
    Specification
    CHO cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Mrs. Soraia Martins

    Verified customer

    Submitted Mar 16 2015

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (12%)
    Sample
    Human Cell lysate - whole cell (Cancer cell)
    Specification
    Cancer cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Hyungtaek Jeon

    Verified customer

    Submitted Nov 06 2014

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Human Serum (Purified exosomes from human serum)
    Specification
    Purified exosomes from human serum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 20 2014

    Application
    Western blot
    Loading amount
    1e+006 cells
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cell)
    Specification
    mouse embryonic stem cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

    Abcam user community

    Verified customer

    Submitted Jan 21 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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