Recombinant Anti-CD81 antibody [EPR4244] (ab109201)
![Western blot - Anti-CD81 antibody [EPR4244] (ab109201)](https://www.abcam.com/ps/products/109/ab109201/Images/ab109201-480634-anti-cd81-antibody-western-blot-human-mouse.jpg "Western blot - Anti-CD81 antibody [EPR4244] (ab109201)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4244] to CD81
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD81 antibody [EPR4244]
See all CD81 primary antibodies -
Description
Rabbit monoclonal [EPR4244] to CD81 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos, JAR, U87-MG, IM-9, peripheral blood mononuclear cell (PBMC), C6, PC-12, C2C12, mouse brain, mouse heart, A549, 293T, HeLa, Huh7, HCT 116, LNCaP, U-87 and RAW264.7 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4244 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
For unpurified use at 1/500 - 1/1000. For WB related protocols, please refer to the Protocols section below. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. For unpurified use at 1/500 - 1/1000. For WB related protocols, please refer to the Protocols section below. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
Tissue specificity
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
Involvement in disease
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsNot glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 975 Human
- Entrez Gene: 12520 Mouse
- Entrez Gene: 25621 Rat
- Omim: 186845 Human
- SwissProt: P60033 Human
- SwissProt: P35762 Mouse
- SwissProt: Q62745 Rat
- Unigene: 54457 Human
see all -
Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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Western blot - Anti-CD81 antibody [EPR4244] (ab109201)All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution
Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with NFDM/TBST
Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysate with NFDM/TBST
Lane 3 : Mouse brain tissue lysate with NFDM/TBST
Lane 4 : Mouse heart tissue lysate with NFDM/TBST
Lane 5 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 6 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate with NFDM/TBST
Lane 7 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 8 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 9 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 10 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 11 : A549 (Human lung carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 12 : 293T (Human embryonic kidney epithelial cell) whole cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 26 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 60 seconds -
![Western blot - Anti-CD81 antibody [EPR4244] (ab109201)](https://www.abcam.com/ps/products/109/ab109201/Images/ab109201-240115-anti-cd81-antibody-epr4244-western-blot.jpg)
Western blot - Anti-CD81 antibody [EPR4244] (ab109201)All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/700 dilution (unpurified)
Lane 1 : Ramos cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : Raw264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-CD81 antibody [EPR4244] (ab109201)](https://www.abcam.com/ps/products/109/ab109201/Images/ab109201-240116-anti-cd81-antibody-epr4244-western-blot.jpg)
Western blot - Anti-CD81 antibody [EPR4244] (ab109201)All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/2000 dilution (purified)
Lane 1 : Ramos cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : Raw264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-CD81 antibody [EPR4244] (ab109201)](https://www.abcam.com/ps/products/109/ab109201/Images/ab109201-121827-anti-cd81-antibody-epr4244-western-blot.jpg)
Western blot - Anti-CD81 antibody [EPR4244] (ab109201)All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution (unpurified)
Lane 1 : Ramos cell lysate
Lane 2 : JAR cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : IM-9 cell lysate
Lane 5 : Peripheral blood mononuclear cell (PBMC) cell lysate
Lane 6 : C6 cell lysate
Lane 7 : PC-12 cell lysate
Lane 8 : RAW264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
Predicted band size: 26 kDa -
![Anti-CD81 antibody [EPR4244] (ab109201)](https://www.abcam.com/ps/products/109/ab109201/Images/ab109201-5-benefits-of-recombinant-antibodies.png)
Anti-CD81 antibody [EPR4244] (ab109201)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (132)
ab109201 has been referenced in 132 publications.
- Zhong Y et al. Circular RNA circ_0032821 contributes to oxaliplatin (OXA) resistance of gastric cancer cells by regulating SOX9 via miR-515-5p. Biotechnol Lett 43:339-351 (2021). PubMed: 33123829
- Ding X et al. Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer. Cancer Manag Res 13:1-11 (2021). PubMed: 33442289
- Wu SC et al. Subpopulations of exosomes purified via different exosomal markers carry different microRNA contents. Int J Med Sci 18:1058-1066 (2021). PubMed: 33456364
- Gao L et al. Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis. J Transl Med 19:37 (2021). PubMed: 33472665
- Albino D et al. Circulating extracellular vesicles release oncogenic miR-424 in experimental models and patients with aggressive prostate cancer. Commun Biol 4:119 (2021). PubMed: 33500545