Anti-CD81 antibody [EPR4244] (ab109201)
Rabbit recombinant monoclonal CD81 antibody [EPR4244]. Validated in WB and tested in Mouse, Rat, Human. Cited in 7 publication(s). Independently reviewed in 4 review(s).
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-CD81 antibody [EPR4244]
See all CD81 primary antibodies -
DescriptionRabbit monoclonal [EPR4244] to CD81
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human CD81 aa 150-250. The exact sequence is proprietary.
Database link: P60033 -
Positive control
- WB: Ramos, JAR, U87-MG, IM-9, peripheral blood mononuclear cell (PBMC), C6, PC-12, and RAW264.7 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR4244
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab109201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 26 kDa. For unpurified use at 1/500 - 1/1000. |
Target
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FunctionMay play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
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Tissue specificityHematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
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Involvement in diseaseDefects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
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Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
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Post-translational
modificationsNot glycosylated. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 975 Human
- Entrez Gene: 12520 Mouse
- Entrez Gene: 25621 Rat
- Omim: 186845 Human
- SwissProt: P60033 Human
- SwissProt: P35762 Mouse
- SwissProt: Q62745 Rat
- Unigene: 54457 Human
see all -
Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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![Western blot - Anti-CD81 antibody [EPR4244] (ab109201)](https://www.abcam.com/ps/products/109/ab109201/Images/ab109201-240115-ab109201upwb.jpg)
Western blot - Anti-CD81 antibody [EPR4244] (ab109201)All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/700 dilution (unpurified)
Lane 1 : Ramos cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : Raw264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-CD81 antibody [EPR4244] (ab109201)](https://www.abcam.com/ps/products/109/ab109201/Images/ab109201-240116-ab109201pwb.jpg)
Western blot - Anti-CD81 antibody [EPR4244] (ab109201)All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/2000 dilution (purified)
Lane 1 : Ramos cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : Raw264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-CD81 antibody [EPR4244] (ab109201)](https://www.abcam.com/ps/products/109/ab109201/Images/TAPA1-Primary-antibodies-ab109201-1.JPG)
Western blot - Anti-CD81 antibody [EPR4244] (ab109201)All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution (unpurified)
Lane 1 : Ramos cell lysate
Lane 2 : JAR cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : IM-9 cell lysate
Lane 5 : Peripheral blood mononuclear cell (PBMC) cell lysate
Lane 6 : C6 cell lysate
Lane 7 : PC-12 cell lysate
Lane 8 : RAW264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)
Predicted band size: 26 kDa
Protocols
References
This product has been referenced in:
- Dong H et al. Exosome-mediated transfer of lncRNA-SNHG14 promotes trastuzumab chemoresistance in breast cancer. Int J Oncol 53:1013-1026 (2018). Read more (PubMed: 30015837) »
- Domenis R et al. Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm 2017:4814987 (2017). WB ; Human . Read more (PubMed: 28634420) »
