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    cd81-antibody-epr4244-ab109201.pdf

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RecombinantRabMAb

Recombinant Anti-CD81 antibody [EPR4244] (ab109201)

  • Datasheet
  • SDS
Reviews (6)Q&A (11)References (132)

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Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
  • Anti-CD81 antibody [EPR4244] (ab109201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4244] to CD81
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-CD81 antibody [EPR4244]
    See all CD81 primary antibodies
  • Description

    Rabbit monoclonal [EPR4244] to CD81
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Ramos, JAR, U87-MG, IM-9, peripheral blood mononuclear cell (PBMC), C6, PC-12, C2C12, mouse brain, mouse heart, A549, 293T, HeLa, Huh7, HCT 116, LNCaP, U-87 and RAW264.7 cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4244
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Microbiology
    • Protein
    • Human Protein
    • Receptor

Associated products

  • Alternative Versions

    • Anti-CD81 antibody [EPR4244] - BSA and Azide free (ab232390)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Ramos whole cell lysate (ab3955)
    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant Human CD81 protein (Fc Chimera His Tag) (ab226886)
    • Recombinant Human CD81 protein (Tagged) (ab267988)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (6)
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.

For unpurified use at 1/500 - 1/1000.

For WB related protocols, please refer to the Protocols section below.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.

For unpurified use at 1/500 - 1/1000.

For WB related protocols, please refer to the Protocols section below.

Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.
  • Tissue specificity

    Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
  • Involvement in disease

    Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications

    Not glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P60033 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 975 Human
    • Entrez Gene: 12520 Mouse
    • Entrez Gene: 25621 Rat
    • Omim: 186845 Human
    • SwissProt: P60033 Human
    • SwissProt: P35762 Mouse
    • SwissProt: Q62745 Rat
    • Unigene: 54457 Human
    • Unigene: 806 Mouse
    • Unigene: 1975 Rat
    see all
  • Alternative names

    • 26 kDa cell surface protein TAPA 1 antibody
    • 26 kDa cell surface protein TAPA-1 antibody
    • 26 kDa cell surface protein TAPA1 antibody
    • CD 81 antibody
    • CD81 antibody
    • CD81 antigen (target of antiproliferative antibody 1) antibody
    • CD81 antigen antibody
    • CD81 molecule antibody
    • CD81_HUMAN antibody
    • CVID6 antibody
    • S5.7 antibody
    • TAPA 1 antibody
    • TAPA1 antibody
    • Target of the antiproliferative antibody 1 antibody
    • Tetraspanin 28 antibody
    • Tetraspanin-28 antibody
    • Tetraspanin28 antibody
    • Tspan 28 antibody
    • Tspan-28 antibody
    • Tspan28 antibody
    see all

Images

  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
    Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
    All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with NFDM/TBST
    Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysate with NFDM/TBST
    Lane 3 : Mouse brain tissue lysate with NFDM/TBST
    Lane 4 : Mouse heart tissue lysate with NFDM/TBST
    Lane 5 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with NFDM/TBST
    Lane 6 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate with NFDM/TBST
    Lane 7 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with NFDM/TBST
    Lane 8 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate with NFDM/TBST
    Lane 9 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate with NFDM/TBST
    Lane 10 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with NFDM/TBST
    Lane 11 : A549 (Human lung carcinoma epithelial cell) whole cell lysate with NFDM/TBST
    Lane 12 : 293T (Human embryonic kidney epithelial cell) whole cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 26 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?


    Exposure time: 60 seconds
  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
    Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
    All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/700 dilution (unpurified)

    Lane 1 : Ramos cell lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : Raw264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 26 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
    Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
    All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/2000 dilution (purified)

    Lane 1 : Ramos cell lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : Raw264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 26 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
    Western blot - Anti-CD81 antibody [EPR4244] (ab109201)
    All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution (unpurified)

    Lane 1 : Ramos cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : IM-9 cell lysate
    Lane 5 : Peripheral blood mononuclear cell (PBMC) cell lysate
    Lane 6 : C6 cell lysate
    Lane 7 : PC-12 cell lysate
    Lane 8 : RAW264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

    Predicted band size: 26 kDa

  • Anti-CD81 antibody [EPR4244] (ab109201)
    Anti-CD81 antibody [EPR4244] (ab109201)

Protocols

  • Western blot protocol
  • Lysate preparation for Western blot

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (132)

Publishing research using ab109201? Please let us know so that we can cite the reference in this datasheet.

ab109201 has been referenced in 132 publications.

  • Zhong Y  et al. Circular RNA circ_0032821 contributes to oxaliplatin (OXA) resistance of gastric cancer cells by regulating SOX9 via miR-515-5p. Biotechnol Lett 43:339-351 (2021). PubMed: 33123829
  • Ding X  et al. Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer. Cancer Manag Res 13:1-11 (2021). PubMed: 33442289
  • Wu SC  et al. Subpopulations of exosomes purified via different exosomal markers carry different microRNA contents. Int J Med Sci 18:1058-1066 (2021). PubMed: 33456364
  • Gao L  et al. Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis. J Transl Med 19:37 (2021). PubMed: 33472665
  • Albino D  et al. Circulating extracellular vesicles release oncogenic miR-424 in experimental models and patients with aggressive prostate cancer. Commun Biol 4:119 (2021). PubMed: 33500545
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Western blot abreview for Anti-CD81 antibody [EPR4244]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hMSC and extrecellular vesicles)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
10 µg
Specification
hMSC and extrecellular vesicles
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 22 2021

Western blot abreview for Anti-CD81 antibody [EPR4244]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Melanoma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 13 2021

Western blot abreview for Anti-TAPA1 antibody [EPR4244]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Hamster Cell lysate - whole cell (CHO cells)
Specification
CHO cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Soraia Martins

Verified customer

Submitted Mar 16 2015

Western blot abreview for Anti-TAPA1 antibody [EPR4244]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12%)
Sample
Human Cell lysate - whole cell (Cancer cell)
Specification
Cancer cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Hyungtaek Jeon

Verified customer

Submitted Nov 06 2014

Western blot abreview for Anti-TAPA1 antibody [EPR4244]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Serum (Purified exosomes from human serum)
Specification
Purified exosomes from human serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 20 2014

Western blot abreview for Anti-TAPA1 antibody [EPR4244]

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 21 2014

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