Recombinant Anti-CD81 antibody [EPR4244] - BSA and Azide free (ab232390)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4244] to CD81 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD81 antibody [EPR4244] - BSA and Azide free
See all CD81 primary antibodies -
Description
Rabbit monoclonal [EPR4244] to CD81 - BSA and Azide free -
Host species
Rabbit -
Specificity
To detect stronger signal in exosome lysate with this antibody, please try protocols of exosome isolation in PMID: 33184263, PMID: 34502537 , PMID: 32121214, PMID: 32375124.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Ramos, JAR, U87-MG, IM-9, peripheral blood mononuclear cell (PBMC), C6, PC-12, C2C12, mouse brain, mouse heart, A549, 293T, HeLa, Huh7, HCT 116, LNCaP, U-87 and RAW264.7 cell lysates.
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General notes
ab232390 is the carrier-free version of ab109201.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4244 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
For WB related protocols, please refer to the Protocols section below. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. For WB related protocols, please refer to the Protocols section below. |
Target
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Function
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
Tissue specificity
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
Involvement in disease
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsNot glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 975 Human
- Entrez Gene: 12520 Mouse
- Entrez Gene: 25621 Rat
- Omim: 186845 Human
- SwissProt: P60033 Human
- SwissProt: P35762 Mouse
- SwissProt: Q62745 Rat
- Unigene: 54457 Human
see all -
Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution
Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with NFDM/TBST
Lane 2 : C2C12 (Mouse myoblasts myoblast) whole cell lysate with NFDM/TBST
Lane 3 : Mouse brain tissue lysate with NFDM/TBST
Lane 4 : Mouse heart tissue lysate with NFDM/TBST
Lane 5 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 6 : HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate with NFDM/TBST
Lane 7 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 8 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 9 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 10 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 11 : A549 (Human lung carcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 12 : 293T (Human embryonic kidney epithelial cell) whole cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 60 seconds -
All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/700 dilution (unpurified)
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109201).
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab232390 has been referenced in 2 publications.
- Adduri RSR et al. Realistic biomarkers from plasma extracellular vesicles for detection of beryllium exposure. Int Arch Occup Environ Health 95:1785-1796 (2022). PubMed: 35551477
- Zou L et al. Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy. Exp Ther Med 18:2574-2582 (2019). PubMed: 31555366