Key features and details
- Rabbit polyclonal to CD82
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD82 antibody
See all CD82 primary antibodies
DescriptionRabbit polyclonal to CD82
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD82 (C terminal). The exact sequence is proprietary. Carrier protein conjugated
Database link: P27701
- WB: K562 whole cell lysate. IHC-P: Human breast cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab262695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Tissue specificityLymphoid specific.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CD82 using ab262695 at 1/250 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15mins.
Anti-CD82 antibody (ab262695) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 30 µg
Predicted band size: 29 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab262695 has not yet been referenced specifically in any publications.