Anti-CD82 antibody [B-L2] - BSA and Azide free (ab47153)
Key features and details
- Mouse monoclonal [B-L2] to CD82 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD82 antibody [B-L2] - BSA and Azide free
See all CD82 primary antibodies -
Description
Mouse monoclonal [B-L2] to CD82 - BSA and Azide free -
Host species
Mouse -
Specificity
Recognises the CD82 antigen, a 60 kDa protein. Cross reacts with mouse CD82 protein in cytometry.
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Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD82. CD82 transfected P815 cell line
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
Carrier free
Yes -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
Sterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free. -
Clonality
Monoclonal -
Clone number
B-L2 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. -
Tissue specificity
Lymphoid specific. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3732 Human
- Omim: 600623 Human
- SwissProt: P27701 Human
- Unigene: 527778 Human
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Alternative names
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
see all
Images
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A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes
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IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab47153 has been referenced in 3 publications.
- Di Giacomo V et al. ?Np63a promotes adhesion of metastatic prostate cancer cells to the bone through regulation of CD82. Oncogene 36:4381-4392 (2017). Flow Cyt . PubMed: 28368419
- Marjon KD et al. Tetraspanin CD82 regulates bone marrow homing of acute myeloid leukemia by modulating the molecular organization of N-cadherin. Oncogene 35:4132-40 (2016). ICC/IF . PubMed: 26592446
- Artavanis-Tsakonas K et al. The Tetraspanin CD82 is Specifically Recruited to Fungal and Bacterial Phagosomes Prior to Acidification. Infect Immun : (2010). ICC/IF ; Human . PubMed: 21149584