Overview

  • Product name

    Anti-CD82 antibody [B-L2]
    See all CD82 primary antibodies
  • Description

    Mouse monoclonal [B-L2] to CD82
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    CD82 transfected P815 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab47153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
  • Tissue specificity

    Lymphoid specific.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4F9 antibody
    • Antigen detected by monoclonal and antibody IA4 antibody
    • C33 antibody
    • C33 antigen antibody
    • CD 82 antibody
    • CD82 antibody
    • CD82 antigen antibody
    • CD82 molecule antibody
    • CD82_HUMAN antibody
    • GR 15 antibody
    • GR15 antibody
    • IA 4 antibody
    • IA4 antibody
    • Inducible membrane protein antibody
    • Inducible membrane protein R2 antibody
    • KAI 1 antibody
    • KAI1 antibody
    • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
    • Kangai 1 antibody
    • Kangai1 antibody
    • Leukocyte surface antigen R2 antibody
    • Metastasis suppressor Kangai 1 antibody
    • Metastasis suppressor Kangai-1 antibody
    • Metastasis suppressor Kangai1 antibody
    • Prostate cancer antimetastasis gene KAI1 antibody
    • R2 antibody
    • R2 leukocyte antigen antibody
    • SAR 2 antibody
    • SAR2 antibody
    • ST 6 antibody
    • ST6 antibody
    • Suppression of tumorigenicity 6 antibody
    • Suppression of tumorigenicity 6 prostate antibody
    • Suppressor of tumorigenicity 6 antibody
    • Suppressor of tumorigenicity 6 protein antibody
    • Tetraspanin 27 antibody
    • Tetraspanin-27 antibody
    • Tetraspanin27 antibody
    • Tspan 27 antibody
    • Tspan-27 antibody
    • Tspan27 antibody
    see all

Images

  • ab47153 staining HeLa cells.

    ICC/IF was performed with a standard paraformaldehyde technique (non permeabilized, blocked with 0.2% fish gelatin for 30 min at 25°C). Primary antibody used at 1/200 in 0.12% PBS / fish gelatin for one hour.  The secondary antibody was a Cy3-conjugated Goat anti-Mouse. 

    See Abreview

  • A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes
  • IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Di Giacomo V  et al. ?Np63a promotes adhesion of metastatic prostate cancer cells to the bone through regulation of CD82. Oncogene 36:4381-4392 (2017). Flow Cyt . Read more (PubMed: 28368419) »
  • Marjon KD  et al. Tetraspanin CD82 regulates bone marrow homing of acute myeloid leukemia by modulating the molecular organization of N-cadherin. Oncogene 35:4132-40 (2016). ICC/IF . Read more (PubMed: 26592446) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer


I am sorry to confirm that the epitope for these antibodies have not been mapped. This is not often done for research grade antibodies.

I can confirm that the immunisation was done with transfected cells. So the immunogen was the full sequence, expressed at the membrane.

Read More

Answer

Thank you for your reply.


We currently only have GR45737-2 and GR45737-3 in stock. We have sold out of GR45737-1. However, these are all the same antibody and are all from the same parent lot, just aliquoted at different times. If you would like to order a specific lot, you'll have to mention that to customer service when ordering. Lot GR45737-2 would ship from our US office and GR45737-3 would ship from our UK office.


Please let me know if you have any questions.

Read More

Answer

Thank you for your inquiry.


Unfortunately, we have not tested, nor do we guarantee, this antibody to work in blocking protein-protein interactions. If we had, we would list that on the online datasheet as "blocking," "functional studies," or "neutralizing." In this case, this antibody has only been tested in IHC-P, IHC-Fr, ICC/IF, andFlow Cyt.


Since the antibody is being used in an untested application, we cannot offer a replacement or refund in accordance with our Abpromise.

https://www.abcam.com/index.html?pageconfig=resource&rid=10372


It looks like none of our anti-CD82 antibodies have been tested for their blocking ability and the only lots we have in stock for ab47153 are from the same parent lot as GR45737-3.


I see that you have ordered this antibody 4 times in the past. You've tried lot GR45737-1and GR45737-3. These are from the same parent lot. The number after the dash just describes the order in which it was aliquoted. I do not know why, then, that you would be seeing different results as these are in fact from the same parent lot of antibody.


I hope this information helps. Please contact us with any other questions.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
fish gelatin as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 25°C

Dr. Lydia Danglot

Verified customer

Submitted Nov 13 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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