For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I have been using anti Kai1 antibody (Anti-CD82 antibody [B-L2] - Azide free (ab47153)) for quite some time. I use it to block the interaction of the protein with another protein in cell culture.
The antibody was working fine until I received the last two vials. They both have the same lot number: GR45737-3. I ordered the second vial cause the first one didn’t work but now I found out that the second one doesn’t work either.
Would you please be able to help me to either find out whether there is something wrong with this batch or perhaps you could send me another vial that I can try? I have been ordering quite a lot of this particular antibody and never had a problem until only recently and because I need it quite urgently your prompt response is highly appreciated.
Asked on Mar 14 2012
Thank you for your inquiry.
Unfortunately, we have not tested, nor do we guarantee, this antibody to work in blocking protein-protein interactions. If we had, we would list that on the online datasheet as "blocking," "functional studies," or "neutralizing." In this case, this antibody has only been tested in IHC-P, IHC-Fr, ICC/IF, andFlow Cyt.
Since the antibody is being used in an untested application, we cannot offer a replacement or refund in accordance with our Abpromise.
It looks like none of our anti-CD82 antibodies have been tested for their blocking ability and the only lots we have in stock for ab47153 are from the same parent lot as GR45737-3.
I see that you have ordered this antibody 4 times in the past. You've tried lot GR45737-1and GR45737-3. These are from the same parent lot. The number after the dash just describes the order in which it was aliquoted. I do not know why, then, that you would be seeing different results as these are in fact from the same parent lot of antibody.
I hope this information helps. Please contact us with any other questions.
Answered on Mar 14 2012