Anti-CD82 antibody [B-L2] - BSA and Azide free (ab269718)
![Flow Cytometry - Anti-CD82 antibody [B-L2] - BSA and Azide free (ab269718)](https://www.abcam.com/ps/products/269/ab269718/Images/ab269718-3-59506anticd82antibodybl2flowcytometry.jpg "Flow Cytometry - Anti-CD82 antibody [B-L2] - BSA and Azide free (ab269718)")
Key features and details
- Mouse monoclonal [B-L2] to CD82 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD82 antibody [B-L2] - BSA and Azide free
See all CD82 primary antibodies -
Description
Mouse monoclonal [B-L2] to CD82 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD82. CD82 transfected P815 cell line
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General notes
ab269718 is a carrier free version of ab47153.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
Sterile-filtered through .22 µm and treated to remove endotoxins. Carrier and preservative free. -
Clonality
Monoclonal -
Clone number
B-L2 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC/IF |
Use at an assay dependent concentration.
|
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. -
Tissue specificity
Lymphoid specific. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3732 Human
- Omim: 600623 Human
- SwissProt: P27701 Human
- Unigene: 527778 Human
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Alternative names
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
see all
Images
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Flow Cytometry - Anti-CD82 antibody [B-L2] - BSA and Azide free (ab269718)
A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47153).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody (ab269718)IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47153). -
Immunocytochemistry/ Immunofluorescence - Anti-CD82 antibody (ab269718)ab47153 staining HeLa cells.
ICC/IF was performed with a standard paraformaldehyde technique (non permeabilized, blocked with 0.2% fish gelatin for 30 min at 25°C). Primary antibody used at 1/200 in 0.12% PBS / fish gelatin for one hour. The secondary antibody was a Cy3-conjugated Goat anti-Mouse.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47153).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab269718 has not yet been referenced specifically in any publications.
