Anti-CD82 antibody [B-L2] - BSA and Azide free (ab47153)

Reviews (1)Q&A (3)References (3)
Flow Cytometry - Anti-CD82 antibody [B-L2] (ab47153)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [B-L2] (ab47153)

Key features and details

  • Mouse monoclonal [B-L2] to CD82 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD82 antibody [B-L2] - BSA and Azide free
    See all CD82 primary antibodies

  • Description

    Mouse monoclonal [B-L2] to CD82 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human CD82. CD82 transfected P815 cell line

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab47153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.

  • Tissue specificity

    Lymphoid specific.

  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 4F9 antibody
      • Antigen detected by monoclonal and antibody IA4 antibody
      • C33 antibody
      • C33 antigen antibody
      • CD 82 antibody
      • CD82 antibody
      • CD82 antigen antibody
      • CD82 molecule antibody
      • CD82_HUMAN antibody
      • GR 15 antibody
      • GR15 antibody
      • IA 4 antibody
      • IA4 antibody
      • Inducible membrane protein antibody
      • Inducible membrane protein R2 antibody
      • KAI 1 antibody
      • KAI1 antibody
      • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
      • Kangai 1 antibody
      • Kangai1 antibody
      • Leukocyte surface antigen R2 antibody
      • Metastasis suppressor Kangai 1 antibody
      • Metastasis suppressor Kangai-1 antibody
      • Metastasis suppressor Kangai1 antibody
      • Prostate cancer antimetastasis gene KAI1 antibody
      • R2 antibody
      • R2 leukocyte antigen antibody
      • SAR 2 antibody
      • SAR2 antibody
      • ST 6 antibody
      • ST6 antibody
      • Suppression of tumorigenicity 6 antibody
      • Suppression of tumorigenicity 6 prostate antibody
      • Suppressor of tumorigenicity 6 antibody
      • Suppressor of tumorigenicity 6 protein antibody
      • Tetraspanin 27 antibody
      • Tetraspanin-27 antibody
      • Tetraspanin27 antibody
      • Tspan 27 antibody
      • Tspan-27 antibody
      • Tspan27 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD82 antibody [B-L2] (ab47153)
      Flow Cytometry - Anti-CD82 antibody [B-L2] (ab47153)
      A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [B-L2] (ab47153)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody [B-L2] (ab47153)
      IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (3)

    Publishing research using ab47153? Please let us know so that we can cite the reference in this datasheet.

    ab47153 has been referenced in 3 publications.

    • Di Giacomo V  et al. ?Np63a promotes adhesion of metastatic prostate cancer cells to the bone through regulation of CD82. Oncogene 36:4381-4392 (2017). Flow Cyt . PubMed: 28368419
    • Marjon KD  et al. Tetraspanin CD82 regulates bone marrow homing of acute myeloid leukemia by modulating the molecular organization of N-cadherin. Oncogene 35:4132-40 (2016). ICC/IF . PubMed: 26592446
    • Artavanis-Tsakonas K  et al. The Tetraspanin CD82 is Specifically Recruited to Fungal and Bacterial Phagosomes Prior to Acidification. Infect Immun : (2010). ICC/IF ; Human . PubMed: 21149584

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD82 antibody [B-L2] - Azide free

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells)
    Specification
    HeLa cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    fish gelatin as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Read More

    Dr. Lydia Danglot

    Verified customer

    Submitted Nov 13 2007

    Question

    What is the specific epitope of the B-L2 antibodies (against CD82/KAI1) you provide?

    Read More
    Answer


    I am sorry to confirm that the epitope for these antibodies have not been mapped. This is not often done for research grade antibodies.

    I can confirm that the immunisation was done with transfected cells. So the immunogen was the full sequence, expressed at the membrane.

    Read More

    Question

    Thank you very much for your reply.

    I understand that you dont guarantee the tests that you dont cover in your studies. But it is strange that the antibody didn’t work in my experiments only with the last two vials. Could I please ask if I place an order for the same antibody if it is possible to get a vial from either the same old aliquot that I had before the last two ones, namely GR45737-1, Or if I can at all get a totally new lot number??

    Thanks in advance for your help.

    Read More
    Answer

    Thank you for your reply.


    We currently only have GR45737-2 and GR45737-3 in stock. We have sold out of GR45737-1. However, these are all the same antibody and are all from the same parent lot, just aliquoted at different times. If you would like to order a specific lot, you'll have to mention that to customer service when ordering. Lot GR45737-2 would ship from our US office and GR45737-3 would ship from our UK office.


    Please let me know if you have any questions.

    Read More

    Question

    I have been using anti Kai1 antibody (Anti-CD82 antibody [B-L2] - Azide free (ab47153)) for quite some time. I use it to block the interaction of the protein with another protein in cell culture.

    The antibody was working fine until I received the last two vials. They both have the same lot number: GR45737-3. I ordered the second vial cause the first one didn’t work but now I found out that the second one doesn’t work either.

    Would you please be able to help me to either find out whether there is something wrong with this batch or perhaps you could send me another vial that I can try? I have been ordering quite a lot of this particular antibody and never had a problem until only recently and because I need it quite urgently your prompt response is highly appreciated.

    Read More
    Answer

    Thank you for your inquiry.


    Unfortunately, we have not tested, nor do we guarantee, this antibody to work in blocking protein-protein interactions. If we had, we would list that on the online datasheet as "blocking," "functional studies," or "neutralizing." In this case, this antibody has only been tested in IHC-P, IHC-Fr, ICC/IF, andFlow Cyt.


    Since the antibody is being used in an untested application, we cannot offer a replacement or refund in accordance with our Abpromise.

    https://www.abcam.com/index.html?pageconfig=resource&rid=10372


    It looks like none of our anti-CD82 antibodies have been tested for their blocking ability and the only lots we have in stock for ab47153 are from the same parent lot as GR45737-3.


    I see that you have ordered this antibody 4 times in the past. You've tried lot GR45737-1and GR45737-3. These are from the same parent lot. The number after the dash just describes the order in which it was aliquoted. I do not know why, then, that you would be seeing different results as these are in fact from the same parent lot of antibody.


    I hope this information helps. Please contact us with any other questions.

    Read More

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