Product nameAnti-CD82 antibody [EPR4112]
See all CD82 primary antibodies
DescriptionRabbit monoclonal [EPR4112] to CD82
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to a region within the C-terminal end of the intracellular domain of Human CD82.
- U87 MG, Jurkat, Raji and C6 cell lysates; Human tonsil tissue.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAssociates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Tissue specificityLymphoid specific.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
All lanes : Anti-CD82 antibody [EPR4112] (ab109529) at 1/1000 dilution
Lane 1 : U87 MG cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Raji cell lysate
Lane 4 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa
ab109529, at 1/250 dilution, staining CD82, in paraffin embedded Human tonsil tissue by Immunohistochemistry.
This product has been referenced in:
- Ding C et al. Long non-coding RNA HOTAIR promotes cell migration and invasion via down-regulation of RNA binding motif protein 38 in hepatocellular carcinoma cells. Int J Mol Sci 15:4060-76 (2014). WB ; Human . Read more (PubMed: 24663081) »