Recombinant
RabMAb

Recombinant Anti-CD83 antibody [EPR22405] (ab244204)

Overview

  • Product name

    Anti-CD83 antibody [EPR22405]
    See all CD83 primary antibodies
  • Description

    Rabbit monoclonal [EPR22405] to CD83
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD83 aa 1-150. The exact sequence is proprietary.
    Database link: Q01151

  • Positive control

    • WB: LPS treated THP-1 whole cell lysate. Raji and HDLM-2 whole cell lysate (Treated/untreated with PNGase F). ICC/IF: HDLM-2 cells. Flow Cyt: Human PBMC and HDLM-2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab244204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 23 kDa.
ICC/IF 1/50.
Flow Cyt 1/50.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
    • Tissue specificity

      Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • B cell activation 45kDa cell surface glycoprotein Ig superfamily antibody
      • B cell activation protein antibody
      • B-cell activation protein antibody
      • BL11 antibody
      • BL11 PEN antibody
      • CD83 antibody
      • CD83 antigen (activated B lymphocytes, immunoglobulin superfamily) antibody
      • CD83 antigen antibody
      • CD83 molecule antibody
      • CD83_HUMAN antibody
      • Cell surface glycoprotein antibody
      • Cell surface protein HB15 antibody
      • HB15 antibody
      • hCD83 antibody
      see all

    Images

    • All lanes : Anti-CD83 antibody [EPR22405] (ab244204) at 1/1000 dilution

      Lane 1 : Untreated Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
      Lane 2 : Raji whole cell lysate treated with PNGase F
      Lane 3 : Untreated HDLM-2 (human hodgkin lymphoma) whole cell lysate
      Lane 4 : HDLM-2 whole cell lysate treated with PNGase F

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 23 kDa
      Observed band size: 40-50 kDa
      why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in the literature (PMID: 15320871). The bands above 23 kDa are glycosylated forms.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 26 seconds.

    • All lanes : Anti-CD83 antibody [EPR22405] (ab244204) at 1/1000 dilution

      Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
      Lane 2 : THP-1 treated with 100ng/ml LPS for 3 hours, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 23 kDa



      The molecular weight observed is consistent with what has been described in the literature (PMID: 15320871). The bands above 23 kDa are glycosylated forms.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CD83 with ab244204 at 1/50 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left panel) or anti-CD83 RabMab (Right panel). Then stained with Alexa Fluor® 647-conjugated anti-CD14. The result is consistent with what has been described in the literature. (PMID: 15320871).

      Gated on viable cells.

    • Flow cytometric analysis of HDLM-2 (human Hodgkin lymphoma) cell line labeling CD83 with ab244204 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    • Immunofluorescent analysis of HDLM-2 (human Hodgkin lymphoma) cells fixed with 100% methanol, labeling CD83 with ab244204 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in HDLM-2 cells is observed. We recommend using methanol instead of PFA fixation. The nuclear counter stain is DAPI (blue).
      Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
      The negative control is the secondary antibody only.

    References

    ab244204 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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