Recombinant Anti-CD83 antibody [EPR22405] (ab244204)
![Western blot - Anti-CD83 antibody [EPR22405] (ab244204)](https://www.abcam.com/ps/products/244/ab244204/Images/ab244204-338738-anti-cd83-antibody-epr22405-western-blot.jpg "Western blot - Anti-CD83 antibody [EPR22405] (ab244204)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22405] to CD83
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD83 antibody [EPR22405]
See all CD83 primary antibodies -
Description
Rabbit monoclonal [EPR22405] to CD83 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD83 aa 1-150. The exact sequence is proprietary.
Database link: Q01151 -
Positive control
- WB: LPS treated THP-1 whole cell lysate. Raji and HDLM-2 whole cell lysate (Treated/untreated with PNGase F). ICC/IF: HDLM-2 cells. Flow Cyt: Human PBMC and HDLM-2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22405 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab244204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 23 kDa. | |
| ICC/IF | 1/50. | |
| Flow Cyt | 1/50. |
Target
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Function
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. -
Tissue specificity
Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9308 Human
- Omim: 604534 Human
- SwissProt: Q01151 Human
- Unigene: 595133 Human
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Alternative names
- B cell activation 45kDa cell surface glycoprotein Ig superfamily antibody
- B cell activation protein antibody
- B-cell activation protein antibody
see all
Images
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Western blot - Anti-CD83 antibody [EPR22405] (ab244204)All lanes : Anti-CD83 antibody [EPR22405] (ab244204) at 1/1000 dilution
Lane 1 : Untreated Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
Lane 2 : Raji whole cell lysate treated with PNGase F
Lane 3 : Untreated HDLM-2 (human hodgkin lymphoma) whole cell lysate
Lane 4 : HDLM-2 whole cell lysate treated with PNGase F
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 15320871). The bands above 23 kDa are glycosylated forms.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 26 seconds.
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![Western blot - Anti-CD83 antibody [EPR22405] (ab244204)](https://www.abcam.com/ps/products/244/ab244204/Images/ab244204-338737-anti-cd83-antibody-epr22405-western-blot.jpg)
Western blot - Anti-CD83 antibody [EPR22405] (ab244204)All lanes : Anti-CD83 antibody [EPR22405] (ab244204) at 1/1000 dilution
Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : THP-1 treated with 100ng/ml LPS for 3 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDaThe molecular weight observed is consistent with what has been described in the literature (PMID: 15320871). The bands above 23 kDa are glycosylated forms.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)](https://www.abcam.com/ps/products/244/ab244204/Images/ab244204-338736-anti-cd83-antibody-epr22405-flow-cytometry.jpg)
Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CD83 with ab244204 at 1/50 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left panel) or anti-CD83 RabMab (Right panel). Then stained with Alexa Fluor® 647-conjugated anti-CD14. The result is consistent with what has been described in the literature. (PMID: 15320871).
Gated on viable cells.
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![Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)](https://www.abcam.com/ps/products/244/ab244204/Images/ab244204-338735-anti-cd83-antibody-epr22405-flow-cytometry.jpg)
Flow Cytometry - Anti-CD83 antibody [EPR22405] (ab244204)Flow cytometric analysis of HDLM-2 (human Hodgkin lymphoma) cell line labeling CD83 with ab244204 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] (ab244204)](https://www.abcam.com/ps/products/244/ab244204/Images/ab244204-338734-anti-cd83-antibody-epr22405-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] (ab244204)Immunofluorescent analysis of HDLM-2 (human Hodgkin lymphoma) cells fixed with 100% methanol, labeling CD83 with ab244204 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in HDLM-2 cells is observed. We recommend using methanol instead of PFA fixation. The nuclear counter stain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
The negative control is the secondary antibody only. -
![Anti-CD83 antibody [EPR22405] (ab244204)](https://www.abcam.com/ps/products/244/ab244204/Images/ab244204-6-benefits-of-recombinant-antibodies.png)
Anti-CD83 antibody [EPR22405] (ab244204)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab244204 has not yet been referenced specifically in any publications.
