RecombinantRabMAb

Recombinant Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

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Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
  • Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
  • Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22405] to CD83 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD83 antibody [EPR22405] - BSA and Azide free
    See all CD83 primary antibodies

  • Description

    Rabbit monoclonal [EPR22405] to CD83 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human CD83 aa 1-150. The exact sequence is proprietary.
    Database link: Q01151

  • Positive control

    • ICC/IF: HDLM-2 cells. Flow Cyt: Human PBMC and HDLM-2 cells.

  • General notes

    Ab245986 is the carrier-free version of ab244204. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245986 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab245986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

    • Tissue specificity

      Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • B cell activation 45kDa cell surface glycoprotein Ig superfamily antibody
        • B cell activation protein antibody
        • B-cell activation protein antibody
        • BL11 antibody
        • BL11 PEN antibody
        • CD83 antibody
        • CD83 antigen (activated B lymphocytes, immunoglobulin superfamily) antibody
        • CD83 antigen antibody
        • CD83 molecule antibody
        • CD83_HUMAN antibody
        • Cell surface glycoprotein antibody
        • Cell surface protein HB15 antibody
        • HB15 antibody
        • hCD83 antibody
        see all

      Images

      • Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
        Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

        Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CD83 with ab244204 at 1/50 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti rabbit IgG (Dylight® 488, ab98462), at 1/2000 dilution was used as the secondary antibody.

        Cells were stained with rabbit IgG (Left panel) or anti-CD83 RabMab (Right panel). Then stained with Alexa Fluor® 647-conjugated anti-CD14. The result is consistent with what has been described in the literature. (PMID: 15320871).

        Gated on viable cells.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244204).

      • Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
        Flow Cytometry - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

        Flow cytometric analysis of HDLM-2 (human Hodgkin lymphoma) cell line labeling CD83 with ab244204 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

        Gated on viable cells.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244204).

      • Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
        Immunocytochemistry/ Immunofluorescence - Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

        Immunofluorescent analysis of HDLM-2 (human Hodgkin lymphoma) cells fixed with 100% methanol, labeling CD83 with ab244204 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in HDLM-2 cells. We recommend using methanol instead of PFA fixation. The nuclear counter stain is DAPI (blue).
        Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
        The negative control is the secondary antibody only.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244204).
      • Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)
        Anti-CD83 antibody [EPR22405] - BSA and Azide free (ab245986)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab245986? Please let us know so that we can cite the reference in this datasheet.

      ab245986 has not yet been referenced specifically in any publications.

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