Recombinant
RabMAb

Recombinant Anti-CD84 antibody [EPR8324] (ab137049)

Overview

  • Product name

    Anti-CD84 antibody [EPR8324]
    See all CD84 primary antibodies
  • Description

    Rabbit monoclonal [EPR8324] to CD84
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the extracellular domain of Human CD84 (UniProt ID: Q9UIB8).

  • Positive control

    • Namalwa and Raji cell lysates; Raji cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64-82 kDa (predicted molecular weight: 39 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells.
    • Tissue specificity

      Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Developmental stage

      Expression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division.
    • Domain

      ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A.
    • Post-translational
      modifications

      Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
      N-glycosylated.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD_antigen=CD84 antibody
      • CD84 antibody
      • CD84 antigen antibody
      • CD84 molecule antibody
      • CDw84 antibody
      • Cell surface antigen MAX.3 antibody
      • hCD84 antibody
      • Hly9 beta antibody
      • Hly9-beta antibody
      • Leucocyte differentiation antigen CD84 antibody
      • Leukocyte Antigen antibody
      • Leukocyte antigen CD84 antibody
      • Leukocyte differentiation antigen antibody
      • Leukocyte differentiation antigen CD84 antibody
      • LY9B antibody
      • mCD84 antibody
      • Signaling lymphocytic activation molecule 5 antibody
      • SLAF5_HUMAN antibody
      • SLAM family member 5 antibody
      • SLAMF5 antibody
      see all

    Images

    • All lanes : Anti-CD84 antibody [EPR8324] (ab137049) at 1/1000 dilution

      Lane 1 : Namalwa cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 39 kDa

    • Flow cytometric analysis of Raji cells labelling CD84 with ab137049 at dilution 1/100 (red) or Rabbit IgG negative control (green).

    References

    ab137049 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137049.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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