Recombinant
Recombinant Anti-CD84 antibody [EPR8324] (ab137049)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD84 antibody [EPR8324]
See all CD84 primary antibodies -
Description
Rabbit monoclonal [EPR8324] to CD84 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in the extracellular domain of Human CD84 (UniProt ID: Q9UIB8).
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Positive control
- Namalwa and Raji cell lysates; Raji cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8324 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 64-82 kDa (predicted molecular weight: 39 kDa). | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Application notes
Is unsuitable for ICC,IHC-P or IP.
Target
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Function
Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. -
Tissue specificity
Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Developmental stage
Expression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division. -
Domain
ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
N-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8832 Human
- Omim: 604513 Human
- SwissProt: Q9UIB8 Human
- Unigene: 398093 Human
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Alternative names
- CD_antigen=CD84 antibody
- CD84 antibody
- CD84 antigen antibody
see all
Images
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![Western blot - Anti-CD84 antibody [EPR8324] (ab137049)](https://www.abcam.com/ps/datasheet/images/137/ab137049/CD84-Primary-antibodies-ab137049-4.jpg)
Western blot - Anti-CD84 antibody [EPR8324] (ab137049)All lanes : Anti-CD84 antibody [EPR8324] (ab137049) at 1/1000 dilution
Lane 1 : Namalwa cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 39 kDa -
![Flow Cytometry - Anti-CD84 antibody [EPR8324] (ab137049)](https://www.abcam.com/ps/datasheet/images/137/ab137049/CD84-Primary-antibodies-ab137049-2.jpg)
Flow Cytometry - Anti-CD84 antibody [EPR8324] (ab137049)Flow cytometric analysis of Raji cells labelling CD84 with ab137049 at dilution 1/100 (red) or Rabbit IgG negative control (green).
Datasheets and documents
References
ab137049 has not yet been referenced specifically in any publications.
