Recombinant Anti-CD84 antibody [EPR8324] (ab137049)
![Western blot - Anti-CD84 antibody [EPR8324] (ab137049)](https://www.abcam.com/ps/products/137/ab137049/Images/CD84-Primary-antibodies-ab137049-4.jpg "Western blot - Anti-CD84 antibody [EPR8324] (ab137049)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8324] to CD84
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD84 antibody [EPR8324]
See all CD84 primary antibodies -
Description
Rabbit monoclonal [EPR8324] to CD84 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues in the extracellular domain of Human CD84 (UniProt ID: Q9UIB8).
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Positive control
- Namalwa and Raji cell lysates; Raji cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8324 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 64-82 kDa (predicted molecular weight: 39 kDa).
|
|
| Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 64-82 kDa (predicted molecular weight: 39 kDa). |
|
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells. -
Tissue specificity
Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Developmental stage
Expression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division. -
Domain
ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
N-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8832 Human
- Omim: 604513 Human
- SwissProt: Q9UIB8 Human
- Unigene: 398093 Human
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Alternative names
- CD_antigen=CD84 antibody
- CD84 antibody
- CD84 antigen antibody
see all
Images
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Western blot - Anti-CD84 antibody [EPR8324] (ab137049)All lanes : Anti-CD84 antibody [EPR8324] (ab137049) at 1/1000 dilution
Lane 1 : Namalwa cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 39 kDa -
![Flow Cytometry - Anti-CD84 antibody [EPR8324] (ab137049)](https://www.abcam.com/ps/products/137/ab137049/Images/CD84-Primary-antibodies-ab137049-2.jpg)
Flow Cytometry - Anti-CD84 antibody [EPR8324] (ab137049)Flow cytometric analysis of Raji cells labelling CD84 with ab137049 at dilution 1/100 (red) or Rabbit IgG negative control (green). -
![Anti-CD84 antibody [EPR8324] (ab137049)](https://www.abcam.com/ps/products/137/ab137049/Images/ab137049-3-benefits-of-recombinant-antibodies.png)
Anti-CD84 antibody [EPR8324] (ab137049)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137049 has not yet been referenced specifically in any publications.