Product nameAnti-CD84 antibody [EPR8325]
See all CD84 primary antibodies
DescriptionRabbit monoclonal [EPR8325] to CD84
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CD84 aa 300-400. The exact sequence is proprietary.
- Raji and Namalwa cell lysates; Human spleen and tonsil tissues.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 64-82 kDa (predicted molecular weight: 39 kDa).|
|IHC-P||1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/50 - 1/100.|
FunctionPlays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells.
Tissue specificityPredominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Developmental stageExpression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division.
DomainITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A.
modificationsPhosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
Cellular localizationCell membrane.
- Information by UniProt
- CD_antigen=CD84 antibody
- CD84 antibody
- CD84 antigen antibody
All lanes : Anti-CD84 antibody [EPR8325] (ab131256) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Namalwa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 64-82 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD84 with ab131256 antibody at 1/25 dilution.
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD84 with ab131256 antibody at 1/25 dilution.
This product has been referenced in:
- Szabo K et al. The histopathology of labial salivary glands in primary Sjögren's syndrome: focusing on follicular helper T cells in the inflammatory infiltrates. Mediators Inflamm 2014:631787 (2014). IHC-P ; Human . Read more (PubMed: 25177110) »
- Reindel R et al. CD84 is markedly up-regulated in Kawasaki disease arteriopathy. Clin Exp Immunol 177:203-11 (2014). Read more (PubMed: 24635044) »