• Product name

    Anti-CD84 antibody [EPR8325]
    See all CD84 primary antibodies
  • Description

    Rabbit monoclonal [EPR8325] to CD84
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD84 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Raji and Namalwa cell lysates; Human spleen and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab131256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 64-82 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells.
    • Tissue specificity

      Predominantly expressed in hematopoietic tissues, such as lymph node, spleen and peripheral leukocytes. Expressed in macrophages, B-cells, monocytes, platelets, thymocytes, T-cells and dendritic cells. Highly expressed in memory T-cells.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Developmental stage

      Expression is slightly increased in naive B-cells after the first dividion. By contrast, expression on memory B-cells decreased with each successive division.
    • Domain

      ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A.
    • Post-translational

      Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD_antigen=CD84 antibody
      • CD84 antibody
      • CD84 antigen antibody
      • CD84 molecule antibody
      • CDw84 antibody
      • Cell surface antigen MAX.3 antibody
      • hCD84 antibody
      • Hly9 beta antibody
      • Hly9-beta antibody
      • Leucocyte differentiation antigen CD84 antibody
      • Leukocyte Antigen antibody
      • Leukocyte antigen CD84 antibody
      • Leukocyte differentiation antigen antibody
      • Leukocyte differentiation antigen CD84 antibody
      • LY9B antibody
      • mCD84 antibody
      • Signaling lymphocytic activation molecule 5 antibody
      • SLAF5_HUMAN antibody
      • SLAM family member 5 antibody
      • SLAMF5 antibody
      see all


    • All lanes : Anti-CD84 antibody [EPR8325] (ab131256) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Namalwa cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 39 kDa
      Observed band size: 64-82 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD84 with ab131256 antibody at 1/25 dilution.
    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling CD84 with ab131256 antibody at 1/25 dilution.


    This product has been referenced in:

    • Szabo K  et al. The histopathology of labial salivary glands in primary Sjögren's syndrome: focusing on follicular helper T cells in the inflammatory infiltrates. Mediators Inflamm 2014:631787 (2014). IHC-P ; Human . Read more (PubMed: 25177110) »
    • Reindel R  et al. CD84 is markedly up-regulated in Kawasaki disease arteriopathy. Clin Exp Immunol 177:203-11 (2014). Read more (PubMed: 24635044) »
    See all 2 Publications for this product

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