Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)

Reviews (1) Submit a question References (14)
Flow Cytometry - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)

Key features and details

  • Mouse monoclonal [BU63] to CD86 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

APC FITC PE PE/Cy5® PE/Cy7® PerCP

Overview

  • Product name

    Anti-CD86 antibody [BU63] - BSA and Azide free
    See all CD86 primary antibodies

  • Description

    Mouse monoclonal [BU63] to CD86 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human CD86. ARH-77 (B-lymphoblastoid cell line).
    Database link: P42081

  • Positive control

    • FACS: Human PBMC. IHC-P: Human esophageal tumor tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 0.5-1µg for 106 cells.

Final volume 100 µL.
IHC-P (1)
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt
Use 0.5-1µg for 106 cells.

Final volume 100 µL.
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

  • Tissue specificity

    Expressed by activated B-lymphocytes and monocytes.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activation B7-2 antigen 3 antibody
      • Activation B7-2 antigen antibody
      • B-lymphocyte activation antigen B7-2 2 antibody
      • B-lymphocyte activation antigen B7-2 antibody
      • B7 2 antibody
      • B7 antibody
      • B7-2 antibody
      • B7.2 antibody
      • B70 antibody
      • B72 antibody
      • B72 antigen antibody
      • BU63 antibody
      • CD28 antigen ligand 2 2 antibody
      • CD28 antigen ligand 2 antibody
      • Cd28l2 antibody
      • CD28LG2 antibody
      • CD86 antibody
      • CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
      • CD86 antigen antibody
      • CD86 molecule antibody
      • CD86_HUMAN antibody
      • CLS1 antibody
      • CTLA-4 counter-receptor B7.2 2 antibody
      • CTLA-4 counter-receptor B7.2 2, 3 antibody
      • CTLA-4 counter-receptor B7.2 antibody
      • Early T-cell costimulatory molecule 1 antibody
      • ETC-1 antibody
      • FUN 1 antibody
      • FUN-1 antibody
      • FUN1 antibody
      • LAB72 antibody
      • Ly-58 antibody
      • Ly58 antibody
      • MB7 antibody
      • MB7-2 antibody
      • Membrane glycoprotein antibody
      • MGC34413 antibody
      • T lymphocyte activation antigen CD86 precursor antibody
      • T-lymphocyte activation antigen CD86 antibody
      • TS/A-2 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
      Flow Cytometry - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)

      Flow cytometric analysis of human PBMC's labeling CD86 with ab213044 at 1 µg per 106 cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human esophageal tumor tissue labeling CD86 with ab213044 at 1 µg/mL.

    References (14)

    Publishing research using ab213044? Please let us know so that we can cite the reference in this datasheet.

    ab213044 has been referenced in 14 publications.

    • Candadai AA  et al. Deletion of arginase 2 attenuates neuroinflammation in an experimental model of optic neuritis. PLoS One 16:e0247901 (2021). PubMed: 33735314
    • Salikhova D  et al. Therapeutic Effects of hiPSC-Derived Glial and Neuronal Progenitor Cells-Conditioned Medium in Experimental Ischemic Stroke in Rats. Int J Mol Sci 22:N/A (2021). PubMed: 33946667
    • Yuanyuan L  et al. Myeloid NEMO deficiency promotes tumor immunosuppression partly via MCP1-CCR2 axis. Exp Cell Res 399:112467 (2021). PubMed: 33428904
    • Jia W  et al. Homogeneous polyporus polysaccharide inhibits bladder cancer by polarizing macrophages to M1 subtype in tumor microenvironment. BMC Complement Med Ther 21:150 (2021). PubMed: 34034714
    • Xu M  et al. Baicalin regulates macrophages polarization and alleviates myocardial ischaemia/reperfusion injury via inhibiting JAK/STAT pathway. Pharm Biol 58:655-663 (2020). PubMed: 32649845
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD86 antibody [BU63] - BSA and Azide free

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    SuperBlock as blocking agent for 45 minute(s) · Concentration: 1%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 23 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com