Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)

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Overview

  • Product name

    Anti-CD86 antibody [BU63] - BSA and Azide free
    See all CD86 primary antibodies

  • Description

    Mouse monoclonal [BU63] to CD86 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Tissue, cells or virus corresponding to Human CD86. ARH-77 (B-lymphoblastoid cell line).
    Database link: P42081

  • Positive control

    • FACS: Human PBMC. IHC-P: Human esophageal tumor tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab213044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.

Final volume 100 µL.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

  • Tissue specificity

    Expressed by activated B-lymphocytes and monocytes.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activation B7-2 antigen 3 antibody
      • Activation B7-2 antigen antibody
      • B-lymphocyte activation antigen B7-2 2 antibody
      • B-lymphocyte activation antigen B7-2 antibody
      • B7 2 antibody
      • B7 antibody
      • B7-2 antibody
      • B7.2 antibody
      • B70 antibody
      • B72 antibody
      • B72 antigen antibody
      • BU63 antibody
      • CD28 antigen ligand 2 2 antibody
      • CD28 antigen ligand 2 antibody
      • Cd28l2 antibody
      • CD28LG2 antibody
      • CD86 antibody
      • CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
      • CD86 antigen antibody
      • CD86 molecule antibody
      • CD86_HUMAN antibody
      • CLS1 antibody
      • CTLA-4 counter-receptor B7.2 2 antibody
      • CTLA-4 counter-receptor B7.2 2, 3 antibody
      • CTLA-4 counter-receptor B7.2 antibody
      • Early T-cell costimulatory molecule 1 antibody
      • ETC-1 antibody
      • FUN 1 antibody
      • FUN-1 antibody
      • FUN1 antibody
      • LAB72 antibody
      • Ly-58 antibody
      • Ly58 antibody
      • MB7 antibody
      • MB7-2 antibody
      • Membrane glycoprotein antibody
      • MGC34413 antibody
      • T lymphocyte activation antigen CD86 precursor antibody
      • T-lymphocyte activation antigen CD86 antibody
      • TS/A-2 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
      Flow Cytometry - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)

      Flow cytometric analysis of human PBMC's labeling CD86 with ab213044 at 1 µg per 106 cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)

      Immunohistochemical analysis of formalin fixed, paraffin embedded human esophageal tumor tissue labeling CD86 with ab213044 at 1 µg/mL.

    References

    This product has been referenced in:

    • Tan AHY  et al. Lysine-Specific Histone Demethylase 1A Regulates Macrophage Polarization and Checkpoint Molecules in the Tumor Microenvironment of Triple-Negative Breast Cancer. Front Immunol 10:1351 (2019). Read more (PubMed: 31249575) »
    • Kim SJ  et al. Anti-inflammatory effect of Tauroursodeoxycholic acid in RAW 264.7 macrophages, Bone marrow-derived macrophages, BV2 microglial cells, and spinal cord injury. Sci Rep 8:3176 (2018). Read more (PubMed: 29453346) »
    See all 5 Publications for this product

    Publishing research using ab213044? Please let us know so that we can cite the reference in this datasheet.

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