PE Anti-CD86 antibody [BU63] (ab77226)
Key features and details
- PE Mouse monoclonal [BU63] to CD86
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD86 antibody [BU63]
See all CD86 primary antibodies -
Description
PE Mouse monoclonal [BU63] to CD86 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab77226 reacts with CD86 expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD86. B-lymphoblastoid cell line ARH 77
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General notes
The antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
BU63 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- APC Anti-CD86 antibody [BU63], prediluted (ab134385)
- Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
- PE/Cy7® Anti-CD86 antibody [BU63] (ab233571)
- Anti-CD86 antibody [BU63] (ab234000)
- PE/Cy5® Anti-CD86 antibody [BU63] (ab234226)
- PerCP Anti-CD86 antibody [BU63] (ab77131)
- FITC Anti-CD86 antibody [BU63] (ab77276)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Notes |
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Use 20 µl/100 µl of whole blood or 106 cells in a suspension.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
Tissue specificity
Expressed by activated B-lymphocytes and monocytes. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
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Alternative names
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab77226 has been referenced in 4 publications.
- Giguère JF et al. Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol 78:6222-32 (2004). PubMed: 15163715
- Mauri D et al. Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol 155:118-27 (1995). PubMed: 7541409
- Engel P et al. The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood 84:1402-7 (1994). PubMed: 7520767
- Caux C et al. B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med 180:1841-7 (1994). PubMed: 7525840