Anti-CD86 antibody [BU63], prediluted (Allophycocyanin) (ab134385)

Overview

  • Product name

    Anti-CD86 antibody [BU63], prediluted (Allophycocyanin)
    See all CD86 primary antibodies
  • Description

    Mouse monoclonal [BU63] to CD86, prediluted (Allophycocyanin)
  • Host species

    Mouse
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications

    Suitable for: IP, WB, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    B-lymphoblastoid cell line ARH 77.

  • Positive control

    • Human whole blood.

Properties

Applications

Our Abpromise guarantee covers the use of ab134385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. or 100µl whole blood

Target

  • Function

    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • Tissue specificity

    Expressed by activated B-lymphocytes and monocytes.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation B7-2 antigen 3 antibody
    • Activation B7-2 antigen antibody
    • B-lymphocyte activation antigen B7-2 2 antibody
    • B-lymphocyte activation antigen B7-2 antibody
    • B7 2 antibody
    • B7 antibody
    • B7-2 antibody
    • B7.2 antibody
    • B70 antibody
    • B72 antigen antibody
    • BU63 antibody
    • CD28 antigen ligand 2 2 antibody
    • CD28 antigen ligand 2 antibody
    • Cd28l2 antibody
    • CD28LG2 antibody
    • CD86 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
    • CD86 antigen antibody
    • CD86 molecule antibody
    • CD86_HUMAN antibody
    • CLS1 antibody
    • CTLA-4 counter-receptor B7.2 2, 3 antibody
    • CTLA-4 counter-receptor B7.2 antibody
    • Early T-cell costimulatory molecule 1 antibody
    • ETC-1 antibody
    • FUN 1 antibody
    • FUN-1 antibody
    • LAB72 antibody
    • Ly-58 antibody
    • Ly58 antibody
    • MB7 antibody
    • MB7-2 antibody
    • Membrane glycoprotein antibody
    • MGC34413 antibody
    • T-lymphocyte activation antigen CD86 antibody
    • TS/A-2 antibody
    see all

Images

  • Flow Cytometry analysis of human peripheral blood cells labeling CD86 with Anti-CD86 antibody [BU63], prediluted (Allophycocyanin) (ab134385).
  • ab134385 staining CD86 in Human PBMCs by Flow Cytometry. The sample was incubated with the primary antibody (1/50 in 2% Human serum + 0.5mM EDTA in PBS) for 25 minutes at 4°C.
    Gating Strategy: Lymphocytes.

     

    See Abreview

References

ab134385 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Permeabilization
No
Sample
Human Cell (PBMC)
Specification
PBMC
Gating Strategy
lymphocytes
Preparation
Cell harvesting/tissue preparation method: Ficoll-Hypaque
Sample buffer: PBS

Abcam user community

Verified customer

Submitted Nov 19 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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