APC Anti-CD86 antibody [BU63], prediluted (ab134385)
Key features and details
- APC Mouse monoclonal [BU63] to CD86, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
APC Anti-CD86 antibody [BU63], prediluted
See all CD86 primary antibodies -
Description
APC Mouse monoclonal [BU63] to CD86, prediluted -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD86. B-lymphoblastoid cell line ARH 77.
-
Positive control
- Human whole blood.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading...
-
Purity
Size exclusion -
Purification notes
The antibody is purified by size-exclusion chromatography and adjusted for direct use. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
BU63 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
- Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
- PE/Cy7® Anti-CD86 antibody [BU63] (ab233571)
- Anti-CD86 antibody [BU63] (ab234000)
- PE/Cy5® Anti-CD86 antibody [BU63] (ab234226)
- PerCP Anti-CD86 antibody [BU63] (ab77131)
- PE Anti-CD86 antibody [BU63] (ab77226)
- FITC Anti-CD86 antibody [BU63] (ab77276)
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | (1) |
Use 10µl for 106 cells.
or 100µl whole blood |
Notes |
---|
Flow Cyt
Use 10µl for 106 cells. or 100µl whole blood |
Target
-
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
Tissue specificity
Expressed by activated B-lymphocytes and monocytes. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
-
Alternative names
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
Images
-
Flow Cytometry analysis of human peripheral blood cells labeling CD86 with Anti-CD86 antibody [BU63], prediluted (Allophycocyanin) (ab134385).
-
ab134385 staining CD86 in Human PBMCs by Flow Cytometry. The sample was incubated with the primary antibody (1/50 in 2% Human serum + 0.5mM EDTA in PBS) for 25 minutes at 4°C.
Gating Strategy: Lymphocytes.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab134385 has not yet been referenced specifically in any publications.