Key features and details
- Mouse monoclonal [BU63] to CD86, prediluted (APC)
- Suitable for: IP, WB, IHC-Fr, Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Product nameAnti-CD86 antibody [BU63], prediluted (APC)
See all CD86 primary antibodies
DescriptionMouse monoclonal [BU63] to CD86, prediluted (APC)
ConjugationAPC. Ex: 645nm, Em: 660nm
Tested applicationsSuitable for: IP, WB, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
B-lymphoblastoid cell line ARH 77.
- Human whole blood.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA
Concentration information loading...
Purification notesThe antibody is purified by size-exclusion chromatography and adjusted for direct use. Purity >95% by SDS-PAGE.
- Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
- Anti-CD86 antibody [BU63] (PE/Cy7®) (ab233571)
- Anti-CD86 antibody [BU63] (ab234000)
- Anti-CD86 antibody [BU63] (PE/Cy5®) (ab234226)
- Anti-CD86 antibody [BU63] (PerCP) (ab77131)
- Anti-CD86 antibody [BU63] (PE) (ab77226)
- Anti-CD86 antibody [BU63] (FITC) (ab77276)
Our Abpromise guarantee covers the use of ab134385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 10µl for 106 cells. or 100µl whole blood|
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
- Information by UniProt
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
Flow Cytometry analysis of human peripheral blood cells labeling CD86 with Anti-CD86 antibody [BU63], prediluted (Allophycocyanin) (ab134385).
ab134385 staining CD86 in Human PBMCs by Flow Cytometry. The sample was incubated with the primary antibody (1/50 in 2% Human serum + 0.5mM EDTA in PBS) for 25 minutes at 4°C.
Gating Strategy: Lymphocytes.
ab134385 has not yet been referenced specifically in any publications.