Anti-CD86 antibody [C86/1146] (ab220188)
![Western blot - Anti-CD86 antibody [C86/1146] (ab220188)](https://www.abcam.com/ps/products/220/ab220188/Images/ab220188-277571-anti-cd86-antibody-c86-1146-western-blot.jpg "Western blot - Anti-CD86 antibody [C86/1146] (ab220188)")
Key features and details
- Mouse monoclonal [C86/1146] to CD86
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD86 antibody [C86/1146]
See all CD86 primary antibodies -
Description
Mouse monoclonal [C86/1146] to CD86 -
Host species
Mouse -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein corresponding to Human CD86.
Database link: P42081 -
Positive control
- WB: Daudi cell lysate; IHC-P: Human melanoma tissue; ICC: Raji cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab220188 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
Clonality
Monoclonal -
Clone number
C86/1146 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab220188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use a concentration of 1 - 2 µg/ml. | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For 30 minutes at RT. |
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| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa. |
Target
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Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
Tissue specificity
Expressed by activated B-lymphocytes and monocytes. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 942 Human
- Entrez Gene: 12524 Mouse
- Entrez Gene: 56822 Rat
- Omim: 601020 Human
- SwissProt: P42081 Human
- SwissProt: P42082 Mouse
- Unigene: 171182 Human
- Unigene: 1452 Mouse
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Alternative names
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
Images
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Western blot - Anti-CD86 antibody [C86/1146] (ab220188)Anti-CD86 antibody [C86/1146] (ab220188) at 1 µg + Daudi cell lysate
Predicted band size: 38 kDa
Protocols
Datasheets and documents
References (1)
ab220188 has been referenced in 1 publication.
- Huang H et al. M1 Macrophage Activated by Notch Signal Pathway Contributed to Ventilator-Induced Lung Injury in Chronic Obstructive Pulmonary Disease Model. J Surg Res 244:358-367 (2019). PubMed: 31323391
