Anti-CD86 antibody [C86/1146] (ab220188)

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Overview

  • Product name

    Anti-CD86 antibody [C86/1146]
    See all CD86 primary antibodies

  • Description

    Mouse monoclonal [C86/1146] to CD86

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant full length protein corresponding to Human CD86.
    Database link: P42081

  • Positive control

    • Daudi cell lysate; Human PBMCs; Human melanoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab220188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5-1µg for 106 cells.
IHC-P Use a concentration of 2 - 4 mg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For 30 minutes at RT.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 38 kDa.

Target

  • Function

    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

  • Tissue specificity

    Expressed by activated B-lymphocytes and monocytes.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Activation B7-2 antigen 3 antibody
      • Activation B7-2 antigen antibody
      • B-lymphocyte activation antigen B7-2 2 antibody
      • B-lymphocyte activation antigen B7-2 antibody
      • B7 2 antibody
      • B7 antibody
      • B7-2 antibody
      • B7.2 antibody
      • B70 antibody
      • B72 antibody
      • B72 antigen antibody
      • BU63 antibody
      • CD28 antigen ligand 2 2 antibody
      • CD28 antigen ligand 2 antibody
      • Cd28l2 antibody
      • CD28LG2 antibody
      • CD86 antibody
      • CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
      • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
      • CD86 antigen antibody
      • CD86 molecule antibody
      • CD86_HUMAN antibody
      • CLS1 antibody
      • CTLA-4 counter-receptor B7.2 2 antibody
      • CTLA-4 counter-receptor B7.2 2, 3 antibody
      • CTLA-4 counter-receptor B7.2 antibody
      • Early T-cell costimulatory molecule 1 antibody
      • ETC-1 antibody
      • FUN 1 antibody
      • FUN-1 antibody
      • FUN1 antibody
      • LAB72 antibody
      • Ly-58 antibody
      • Ly58 antibody
      • MB7 antibody
      • MB7-2 antibody
      • Membrane glycoprotein antibody
      • MGC34413 antibody
      • T lymphocyte activation antigen CD86 precursor antibody
      • T-lymphocyte activation antigen CD86 antibody
      • TS/A-2 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD86 antibody [C86/1146] (ab220188)
      Flow Cytometry - Anti-CD86 antibody [C86/1146] (ab220188)

      Flow cytometric analysis of Human PBMC cells labeling CD86 with ab220188 at 1 µg per 106 cells (red), compared with an isotype control (green). 

    • Western blot - Anti-CD86 antibody [C86/1146] (ab220188)
      Western blot - Anti-CD86 antibody [C86/1146] (ab220188)
      Anti-CD86 antibody [C86/1146] (ab220188) at 1 µg + Daudi cell lysate

      Predicted band size: 38 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (ab220188)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [C86/1146] (ab220188)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling CD86 with ab220188 at 4 µg/ml.

    References

    This product has been referenced in:

    • Huang H  et al. M1 Macrophage Activated by Notch Signal Pathway Contributed to Ventilator-Induced Lung Injury in Chronic Obstructive Pulmonary Disease Model. J Surg Res 244:358-367 (2019). Read more (PubMed: 31323391) »
    See 1 Publication for this product

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