Product nameAnti-CD86 antibody [EP1158Y] - N-terminal
See all CD86 primary antibodies
DescriptionRabbit monoclonal [EP1158Y] to CD86 - N-terminal
SpecificityThis antibody has been found to work on mouse samples in WB but not IHC.
Tested applicationsSuitable for: WB, IP, ICC, IHC-P, ICC/IF, ELISA, IHC-Frmore details
Species reactivityReacts with: Human, Cynomolgus monkey
Synthetic peptide within Human CD86 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P42081-1
- WB: Raji cell membrane lysate. IHC-P: Human tonsil tissue. ICC/IF: Raji cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab53004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 70 kDa.|
|ICC||1/100 - 1/250.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21998115|
FunctionReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
Tissue specificityExpressed by activated B-lymphocytes and monocytes.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
- Information by UniProt
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
Pattern of macrophage infiltration in colorectal cancer and healthy surrounding tissues.
The immunoreactivity to CD86 (M1-MF marker, C–D) was analyzed in colorectal cancer (Panel D) and healthy surrounding mucosa (Panel C) using ab53004.
Serial sections (4 µm) of samples containing tumor and normal mucosa from each patient were stained. Endogenous peroxidase activity was suppressed by immersion in 0.3% hydrogen peroxide. Once blocked with 5% horse serum, sections were incubated overnight (4°C) with the primary antibody. A horse anti-mouse/rabbit biotinylated antibody 1∶200 was used as a secondary antibody. The ABC system followed by the DAB were used for development. All tissues were counterstained with hematoxylin.
Immunocytochemistry/Immunofluorescence analysis of Raji (Human Burkitt's lymphoma cell line) labelling CD86 with purified ab53004 at 1/500.
Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI.
PBS was used instead of the primary antibody as the negative control.
Primary antibody, ab53004 was used at concentrations between 0.1 - 1000 ng/ml. An Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1:2500 dilution. Antigen concentration was 100ng/ml.
Anti-CD86 antibody [EP1158Y] - N-terminal (ab53004) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) cell membrane lysate at 10 µg
goat anti-rabbit HRP labeled at 1/2000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?
ab53004 staining CD86 in cynomolgus monkey lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/800) for 30 minutes at room temperature. An undiluted biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ab53004, at a 1/100 dilution, staining human CD86 in tonsil tissue, using Immunohistochemistry, paraffin embedded tissue.
This product has been referenced in:
- Dyck S et al. Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPs receptors promotes a beneficial inflammatory response following spinal cord injury. J Neuroinflammation 15:90 (2018). Read more (PubMed: 29558941) »
- Esposito E et al. Effects of ischemic post-conditioning on neuronal VEGF regulation and microglial polarization in a rat model of focal cerebral ischemia. J Neurochem 146:160-172 (2018). Read more (PubMed: 29570780) »