Recombinant
RabMAb

Anti-CD86 antibody [EP1158Y] - N-terminal (ab53004)

Overview

  • Product name
    Anti-CD86 antibody [EP1158Y] - N-terminal
    See all CD86 primary antibodies
  • Description
    Rabbit monoclonal [EP1158Y] to CD86 - N-terminal
  • Host species
    Rabbit
  • Specificity
    This antibody has been found to work on mouse samples in WB but not IHC.
  • Tested applications
    Suitable for: WB, IP, ICC, IHC-P, ICC/IF, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    Synthetic peptide within Human CD86 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P42081-1

  • Positive control
    • WB: Raji cell membrane lysate. IHC-P: Human tonsil tissue. ICC/IF: Raji cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab53004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 70 kDa.
IP 1/50.
ICC 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21998115

Target

  • Function
    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • Tissue specificity
    Expressed by activated B-lymphocytes and monocytes.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation B7-2 antigen 3 antibody
    • Activation B7-2 antigen antibody
    • B-lymphocyte activation antigen B7-2 2 antibody
    • B-lymphocyte activation antigen B7-2 antibody
    • B7 2 antibody
    • B7 antibody
    • B7-2 antibody
    • B7.2 antibody
    • B70 antibody
    • B72 antigen antibody
    • BU63 antibody
    • CD28 antigen ligand 2 2 antibody
    • CD28 antigen ligand 2 antibody
    • Cd28l2 antibody
    • CD28LG2 antibody
    • CD86 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
    • CD86 antigen antibody
    • CD86 molecule antibody
    • CD86_HUMAN antibody
    • CLS1 antibody
    • CTLA-4 counter-receptor B7.2 2, 3 antibody
    • CTLA-4 counter-receptor B7.2 antibody
    • Early T-cell costimulatory molecule 1 antibody
    • ETC-1 antibody
    • FUN 1 antibody
    • FUN-1 antibody
    • LAB72 antibody
    • Ly-58 antibody
    • Ly58 antibody
    • MB7 antibody
    • MB7-2 antibody
    • Membrane glycoprotein antibody
    • MGC34413 antibody
    • T-lymphocyte activation antigen CD86 antibody
    • TS/A-2 antibody
    see all

Images

  • Pattern of macrophage infiltration in colorectal cancer and healthy surrounding tissues.

    The immunoreactivity to CD86 (M1-MF marker, C–D) was analyzed in colorectal cancer (Panel D) and healthy surrounding mucosa (Panel C) using ab53004.

    Serial sections (4 µm) of samples containing tumor and normal mucosa from each patient were stained. Endogenous peroxidase activity was suppressed by immersion in 0.3% hydrogen peroxide. Once blocked with 5% horse serum, sections were incubated overnight (4°C) with the primary antibody. A horse anti-mouse/rabbit biotinylated antibody 1∶200 was used as a secondary antibody. The ABC system followed by the DAB were used for development. All tissues were counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of Raji (Human Burkitt's lymphoma cell line) labelling CD86 with purified ab53004 at 1/500.

    Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI.

    PBS was used instead of the primary antibody as the negative control.

  • Primary antibody, ab53004 was used at concentrations between 0.1 - 1000 ng/ml. An Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1:2500 dilution. Antigen concentration was 100ng/ml. 

  • Anti-CD86 antibody [EP1158Y] - N-terminal (ab53004) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) cell membrane lysate at 10 µg

    Secondary
    goat anti-rabbit HRP labeled at 1/2000 dilution

    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • ab53004 staining CD86 in cynomolgus monkey lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/800) for 30 minutes at room temperature. An undiluted biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab53004, at a 1/100 dilution, staining human CD86 in tonsil tissue, using Immunohistochemistry, paraffin embedded tissue.

References

This product has been referenced in:
  • Dyck S  et al. Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPs receptors promotes a beneficial inflammatory response following spinal cord injury. J Neuroinflammation 15:90 (2018). Read more (PubMed: 29558941) »
  • Esposito E  et al. Effects of ischemic post-conditioning on neuronal VEGF regulation and microglial polarization in a rat model of focal cerebral ischemia. J Neurochem 146:160-172 (2018). Read more (PubMed: 29570780) »
See all 22 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Antigen retrieval step
None
Specification
Spinal cord
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. CHI ZHANG

Verified customer

Submitted Apr 27 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
Lymph node
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 24 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (spleen)
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Feb 06 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Horse Tissue sections (spleen)
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Feb 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.0
Permeabilization
No
Specification
Lung
Blocking step
(agent) for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 23 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary tumor (MMATV-PyMT))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 5 min high pressure
Permeabilization
Yes - 1mM EDTA, 0.05% Tween 20, pH 8.0
Specification
Mammary tumor (MMATV-PyMT)
Blocking step
5% goat serum +3% BSA in IF buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 14 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
1% BSA + 5% NGS + 0.1% Sapo as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (blood vessels)
Specification
blood vessels
Permeabilization
Yes - saponine
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Sample
Rat Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: Rt°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer pH 6.0
Sample
Cynomolgus Monkey Tissue sections (Lymph node)
Specification
Lymph node
Permeabilization
No
Fixative
Formaldehyde

Jing Ma

Verified customer

Submitted Sep 02 2013

Question
Answer

Thank you for contacting us.

The links to the entry for CD86 in the Human ProteinAtlas are as follows:

http://www.proteinatlas.org/ENSG00000114013
http://www.proteinatlas.org/ENSG00000114013/normal (specifically for the normal tissue)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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