Recombinant Anti-CD86 antibody [EP1158Y] - N-terminal (ab53004)


  • Product name

    Anti-CD86 antibody [EP1158Y] - N-terminal
    See all CD86 primary antibodies
  • Description

    Rabbit monoclonal [EP1158Y] to CD86 - N-terminal
  • Host species

  • Specificity

    This antibody has been found to work on mouse samples in WB but not IHC.
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IF, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    Synthetic peptide within Human CD86 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P42081-1

  • Positive control

    • WB: Raji cell membrane lysate. IHC-P: Human tonsil tissue. ICC/IF: Raji cells.
  • General notes

    We would like to suggest as alternative ab239075 for WB and ICC applications and ab243887 for IHC-P application.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab53004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 70 kDa.
IP 1/50.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21998115


  • Function

    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • Tissue specificity

    Expressed by activated B-lymphocytes and monocytes.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational

    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Activation B7-2 antigen 3 antibody
    • Activation B7-2 antigen antibody
    • B-lymphocyte activation antigen B7-2 2 antibody
    • B-lymphocyte activation antigen B7-2 antibody
    • B7 2 antibody
    • B7 antibody
    • B7-2 antibody
    • B7.2 antibody
    • B70 antibody
    • B72 antibody
    • B72 antigen antibody
    • BU63 antibody
    • CD28 antigen ligand 2 2 antibody
    • CD28 antigen ligand 2 antibody
    • Cd28l2 antibody
    • CD28LG2 antibody
    • CD86 antibody
    • CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
    • CD86 antigen antibody
    • CD86 molecule antibody
    • CD86_HUMAN antibody
    • CLS1 antibody
    • CTLA-4 counter-receptor B7.2 2 antibody
    • CTLA-4 counter-receptor B7.2 2, 3 antibody
    • CTLA-4 counter-receptor B7.2 antibody
    • Early T-cell costimulatory molecule 1 antibody
    • ETC-1 antibody
    • FUN 1 antibody
    • FUN-1 antibody
    • FUN1 antibody
    • LAB72 antibody
    • Ly-58 antibody
    • Ly58 antibody
    • MB7 antibody
    • MB7-2 antibody
    • Membrane glycoprotein antibody
    • MGC34413 antibody
    • T lymphocyte activation antigen CD86 precursor antibody
    • T-lymphocyte activation antigen CD86 antibody
    • TS/A-2 antibody
    see all


  • Pattern of macrophage infiltration in colorectal cancer and healthy surrounding tissues.

    The immunoreactivity to CD86 (M1-MF marker, C–D) was analyzed in colorectal cancer (Panel D) and healthy surrounding mucosa (Panel C) using ab53004.

    Serial sections (4 µm) of samples containing tumor and normal mucosa from each patient were stained. Endogenous peroxidase activity was suppressed by immersion in 0.3% hydrogen peroxide. Once blocked with 5% horse serum, sections were incubated overnight (4°C) with the primary antibody. A horse anti-mouse/rabbit biotinylated antibody 1∶200 was used as a secondary antibody. The ABC system followed by the DAB were used for development. All tissues were counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of Raji (Human Burkitt's lymphoma cell line) labelling CD86 with purified ab53004 at 1/500.

    Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI.

    PBS was used instead of the primary antibody as the negative control.

  • Primary antibody, ab53004 was used at concentrations between 0.1 - 1000 ng/ml. An Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1:2500 dilution. Antigen concentration was 100ng/ml. 

  • Anti-CD86 antibody [EP1158Y] - N-terminal (ab53004) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) cell membrane lysate at 10 µg

    goat anti-rabbit HRP labeled at 1/2000 dilution

    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • ab53004 staining CD86 in cynomolgus monkey lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/800) for 30 minutes at room temperature. An undiluted biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab53004, at a 1/100 dilution, staining human CD86 in tonsil tissue, using Immunohistochemistry, paraffin embedded tissue.


This product has been referenced in:

  • Tyrtyshnaia A  et al. Neuroinflammation and adult hippocampal neurogenesis in neuropathic pain and alkyl glycerol ethers treatment in aged mice. Int J Mol Med 43:2153-2163 (2019). Read more (PubMed: 30896810) »
  • Zhou B  et al. Assisting anti-PD-1 antibody treatment with a liposomal system capable of recruiting immune cells. Nanoscale 11:7996-8011 (2019). Read more (PubMed: 30969294) »
See all 31 Publications for this product

Customer reviews and Q&As


The Human Protein Atlas is a great resource for visualization, quantification and additional references regarding protein expression levels in human cells and tissues.

The Human Protein Atlas entry for CD86 can be found here:

Read More

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